Zeiss Axiovert 200-M for Time-Lapse Microscopy

描述（由申请人提供）：这是一份蔡司倒置显微镜的申请，该显微镜具有精密的电动工作台和灵敏的CCD相机，用于延时活细胞成像。该显微镜将用作专用站，用于使用DIG和荧光光学进行恒焦延时成像。精密的AS)电动压电显微镜台和蔡司环境控制系统将使我们能够在生理条件下连续数小时或数天对细胞进行恒定聚焦成像。这一阶段还将用于准备在同一成像过程中不同位置的细胞的延时序列。灵敏的Photometrics级联CCD相机将显著减少光漂白和维持细胞活力。这个应用程序是基于一个关键的需要，监测细胞反应随着时间的推移，通过延时记录，并将服务于一个大型的nih资助的社区。

DESCRIPTION (provided by applicant): This is an application for a Zeiss inverted microscope with precision motorized stage and sensitive CCD camera for time-lapse, live-cell imaging. The microscope will be used as a dedicated station for constant-focus time-lapse imaging with DIG and fluorescence optics. The precision AS) motorized piezo microscope stage and the Zeiss environmental control system will allow us to image cells at constant focus under physiological conditions for hours or days at a time. This stage will also be used to prepare time-lapse sequences of cells at different locations during the same imaging session. The sensitive Photometrics Cascade CCD camera will significantly reduce photobleaching and maintain cell viability. This application is based on a critical need to monitor cell responses over time by time-lapse recording and will serve a large NIH-funded community.