Zeiss LSM510 Confocal Microscope

描述(由申请人提供)：一个由10名美国国立卫生研究院资助的研究人员组成的小组申请资金购买激光扫描共聚焦显微镜。所要求的系统包括一台蔡司LSM510，配备了可见/近紫外光成像。该小组位于华盛顿州立大学的两个系中，与邻近的爱达荷州大学有研究联系。NIH资助的79名调查人员，总共102名NIH现役补助金位于该地区。该组织与普通研究社区共享使用Biorad MRC1024的机会，目前已进入大量使用的第七个年头。2003年有24家实验室使用了该仪器，从2004年开始，新招募的研究重点是共焦成像的研究人员将增加对该仪器的需求。除了缺乏足够的机会进行长期延时研究外，专家组还受到现有文书无法满足的技术要求的严重限制。其中包括同时对两个以上的荧光探针成像的能力，允许研究活体样本和光敏结构的更高的探测器灵敏度，检测紫外光激发的探测器的能力，以及使用荧光共振能量转移(FRET)测量评估蛋白质-蛋白质相互作用的能力。用户组包括在其他机构拥有蔡司LSM510共焦显微镜系统丰富经验的研究人员，毫无疑问，它有能力满足我们用户组对仪器寿命的需求。该仪器将被安置在一个已建立的名为“EM中心”的多用户成像设施中，该中心目前由两名全职工作人员提供支持。预计请求该仪器的用户组将在75%的时间内使用该仪器，但将在时间允许的情况下提供给非组成员使用。仪器的政策将由内部咨询委员会决定，日常维护、技术监督和指导将由EM中心的工作人员提供。未来5年的服务合同将由充值系统提供，并由兽医、比较解剖学、药理学和生理学系提供财政支持。随着仪器纳入用户组正在进行的研究计划，预计服务协议将在最初的5年期限后通过用户费用维持。

DESCRIPTION (provided by applicant): A group of 10 NIH funded investigators request funds to purchase a laser scanning confocal microscope. The requested system consists of a Zeiss LSM510 equipped for Visible/Near UV imaging. The group is located in two departments at the Washington State University with research ties to the neighboring University of Idaho. 79 NIH funded investigators with a total of 102 active NIH grants are located in the region. The group shares access with the general research community to a BIORAD MRC1024 now in its seventh year of heavy use. 24 laboratories used the instrument in 2003, and newly recruited investigators with research emphasizing confocal imaging will add to the demand for this instrument beginning in 2004. In addition to lack of sufficient access for long-term time-lapse studies, the group is severely limited by technical requirements that cannot be met with the existing instrument. Among these are the ability to image more than two fluorescent probes simultaneously, increased detector sensitivity permitting study of living specimens and photolabile structure, the ability to detect UV excitable probes and the ability to assess protein-protein interaction using fluorescence resonance energy transfer (FRET) measurements. The user group includes investigators with extensive previous experience with the Zeiss LSM510 confocal microscope system at other institutions, and there is no question regarding its ability to serve the needs of our user group for the life-span of the instrument. The instrument will be housed in an established multiuser imaging facility known as the "EM center" which currently offers support by two full time staff members. It is anticipated that the user group requesting the instrument will use the instrument 75% of the time, but provisions will be made for use by non-group members as time permits. Policy for the instrument will be determined by an internal advisory committee, and day-to-day maintenance, technical supervision and instruction will be provided by staff members of the EM center. Service contracts over the next 5 years will be covered by a recharge system and through financial support from the Department of Veterinary, Comparative Anatomy, Pharmacology and Physiology. As the instrument becomes integrated into the user group's ongoing research programs, it is expected that service agreements will be maintained beyond the initial 5 year period by user fees.