TSE Inhalation System

说明(申请人提供)：众所周知，尼古丁和多种致癌物质会使人上瘾，并对健康造成不良影响。然而，烟草的许多不利影响的致病因素尚未确定，其他烟草或烟草烟雾成分可能起到作用。识别和研究这些额外的化合物对于了解烟草相关疾病和成瘾的机制以及评估目前市场上销售的较新的改装卷烟和其他烟草产品的风险非常重要。这些问题是明尼苏达大学(UMN)NIDA/NCI资助的跨学科烟草研究中心(TTURC)的一个具体重点，所要求的仪器将极大地扩大该中心的工作范围。这项建议的具体目的是获得一种TSE吸入系统，以产生香烟烟雾，可用于实验动物直接接触烟草烟雾，制备体外使用的烟草冷凝物，以及对香烟烟雾的个别成分进行定量。研究项目将包括(项目编号1，调查员Hecht)癌症的化学预防和致癌机制，(项目编号2，调查员Murphy)尼古丁代谢，(项目编号3，调查员Pentel)与成瘾相关的行为和孕期吸烟暴露的影响，(项目编号4，调查员Peterson)神经干细胞分化，(项目编号5，调查员Ondrey)上呼吸道消化系癌，以及(项目编号6，调查员贝克泰尔德)骨愈合和植入物整合。TSE吸入系统由自动香烟烟雾发生器、混合室和稀释室、烟雾监测仪器和小动物曝光室组成。可以实时监测和控制烟雾特征和典型的烟雾成分。拥有烟草化学和分析技术经验的核心调查员(赫克特、墨菲、潘特尔)将协助其他调查员获取和鉴定烟草提取物和烟草成分暴露的生物措施。TTURC的核心分析实验室将为这些分析提供便利。TSE系统将设在MMRF，距离明尼苏达大学5分钟车程，将提供空间和所需的设施改造。彭特尔实验室将由一名研究助理监督其运作，该助理将为其他实验室提供服务，包括进行动物暴露实验，并准备烟草冷凝物，然后可根据个别实验室的需要进行进一步加工和表征。这一手段将极大地扩大所涉具体项目的能力和范围。

DESCRIPTION (provided by applicant): Nicotine and a variety of carcinogens are known to contribute to the addictive and adverse health effects of tobacco use. However, the causative agents for many adverse effects of tobacco have not been identified, and additional tobacco or tobacco smoke constituents may contribute. Identifying and studying these additional compounds is important for understanding the mechanisms of tobacco-related disease and addiction, and for assessing the risks of newer modified cigarettes and other tobacco products that are now being marketed. These questions are a specific focus of the University of Minnesota (U of MN) NIDA/NCI-funded Transdisciplinary Tobacco Research Center (TTURC), and the requested instrumentation will substantially expand the scope of the Center's work. The Specific Aim of this proposal is to obtain a TSE Inhalation System to generate cigarette smoke which can be used for the direct exposure of experimental animals to tobacco smoke, for preparation of tobacco condensates for in vitro use, and for quantitation of individual constituents of cigarette smoke. Research projects will include (Project number 1, investigator Hecht) cancer chemoprevention and mechanisms of carcinogenesis, (Project number 2, investigator Murphy) nicotine metabolism, (Project number 3, investigator Pentel) addiction-related behaviors and effects of gestational smoke exposure, (Project number 4, investigator Peterson) neural stem cell differentiation, (Project number 5, investigator Ondrey) upper aerodigestive carcinogenesis, and (Project number 6, investigator Bechtold) bone healing and implant integration. The TSE Inhalation System consists of an automated cigarette smoke generator, mixing and diluting chambers, smoke monitoring instrumentation, and small animal exposure chambers. Smoke characteristics and representative smoke constituents can be monitored and controlled in real time. A core of investigators (Hecht, Murphy, Pentel) with experience in tobacco chemistry and analytical techniques will assist other investigators in obtaining and characterizing tobacco extracts and biological measures of tobacco constituent exposure. The Core Analytical Laboratory of the TTURC will be made available to facilitate these analyses. The TSE system will be housed at MMRF, located 5 minutes from the U of MN, which will provide space and the required facilities modifications. The Pentel laboratory will supervise its operation by a single research assistant who will provide services for the other laboratories, including performing animal exposure experiments, and preparing tobacco condensates which can then be further processed and characterized as needed by the individual laboratories. This instrumentation will considerably expand the capabilities and scope of the specific projects involved.