Automatic Liquid Handling System

描述（由申请人提供）：本申请旨在申请资金支持，用于从TekCel购买自动液体处理系统TekBench-SP，该系统将用于支持我们在西奈山医学院（MSSM）新化学筛选设施的小分子高通量筛选方面的规模扩大工作。这个筛选设施作为新成立的化学生物学中心的一部分，旨在满足大量MSSM研究人员日益增长的需求，以获取和发现新的化学试剂，用作研究探针，研究细胞通路和网络以及细胞成分在健康和疾病中的功能。这种新的机器人仪器取代了我们目前在化学筛选试点项目中使用的Gilson 215单探针液体处理器/注射器。新仪器提供了一个完全集成的自动化液体处理平台，可以自动处理化学筛选所需的各种样品处理应用，包括化学库重新格式化、ELISA、动力学和基于细胞的测定以及樱桃/命中挑选。因此，这种新仪器将极大地促进许多正在进行的NIH资助的西奈山研究人员，他们致力于采用强大的小分子化学生物学方法对广泛的生物系统进行机械研究。

DESCRIPTION (provided by applicant): This application is to request funding support for the purchase of an Automatic Liquid Handling System, TekBench-SP from TekCel, which will be used to support our scale-up efforts in high-throughput screening of small molecules at the new chemical screening facility at Mount Sinai School of Medicine (MSSM). This screening facility as a part of the newly established Chemical Biology Center is created to meet the growing needs of a large number of MSSM's investigators to access and discover new chemical agents to be used as research probes to study cellular pathways and networks and the function of cellular components in health and disease. This new robotic instrument replaces our current Gilson's 215 single-probe liquid handler/injector that we have used in our pilot projects of chemical screening. The new instrument offers a fully integrated and automatic liquid handling platform, and makes it possible for automatic processing of various sample processing applications required for chemical screening, which include chemical library reformatting, ELISA, kinetic and cell-based assays, as well as cherry/hit picking. Thus, this new instrument will greatly facilitate many ongoing NIH funded investigators at Mount Sinai who are committed to employ the powerful chemical biology approach with small molecules to mechanistic investigation of a wide range of biological systems.