IVIS 200 Imaging System

描述(由申请人提供)： 这项应用的主要目标是为我们当地的科学界提供共享的非侵入性光学成像仪器，以提高我们生物医学研究项目的创新、效率和协同效应。我们确定了16名由NIH资助的斯克里普斯研究所、伯纳姆研究所和拉霍亚分子医学研究所的研究人员，他们迫切需要一种成像方法，以便能够在体内监测1)肿瘤的发展和转移进展，2)对肿瘤细胞、干细胞或前体细胞的特定跟踪，以及参与发育、组织重塑、损伤修复和再生的其他细胞类型，3)基因表达，4)蛋白分解活性，以及5)疾病和病理条件下的治疗效果。 作为一种共享仪器，我们提出了一种基于荧光和生物发光信号检测的非侵入性小动物实时成像方法，具有成本效益和易于使用的特点，但仍是最先进的。 斯克里普斯研究所将提供所有辅助设施和工作人员，在收回成本的基础上操作和管理仪器使用和用户培训。它还将提供对生物信息学基础设施的访问，以促进数据分析、管理、存档和与公共数据库的链接。共享仪器的组织和使用将促进用户群体内的研究合作，并为生物医学研究开辟新的途径，增强从每个参与项目中获得的信息，以了解活体动物模型中分子和细胞生物学事件的复杂性。

DESCRIPTION (provided by applicant): The major goal of this application is to provide our local scientific community with access to a shared non-invasive optical imaging instrument to increase the innovation, efficiency, and synergy of our biomedical research projects. We identified sixteen NIH funded investigators of The Scripps Research Institute, the Burnham Institute, and the La Jolla Institute for Molecular Medicine who have an urgent need for an imaging modality that will allow in vivo monitoring of 1) tumor development and metastatic progression, 2) specific tracking of tumor cells, stem or progenitor cells, and other cell types involved in development, tissue remodeling, injury repair and regeneration, 3) gene expression, 4) proteolytic activity, and 5) treatment efficacy in disease and pathological conditions. For use as a shared instrument, we propose a cost effective and easy-to-use, yet state-of-the-art modality for non-invasive real time imaging of small animals, based on the detection of fluorescence and bioluminescence signals. The Scripps Research Institute will provide all ancillary facilities and staff to operate and manage instrument use and user training on a cost recovery basis. It will also provide access to a bioinformatics infrastructure to facilitate data analysis, management, archiving, and links to public databases. The organization and use of the shared instrument will foster research collaborations within the user group and open new avenues for biomedical research, enhancing the information gained from each of the participating projects toward understanding the complexity of molecular and cell biological events in live animal models.