Live 3D airway models incorporating chemical sensors

来自患者细胞的3D组织模型在开发新疗法中越来越重要。3D模型对于设计组织特异性和患者特异性的人类疾病模型至关重要-动物模型无法包含如此多样的基因型，并且并不总是反映人类疾病的工作原理。使用（表面增强拉曼光谱（Sers））的pH和氧化还原电位的非侵入性测量反映了细胞的代谢状态和组织在3D模型中修复和再生的能力，将使治疗的效果能够以目前不可能的方式进行监测。在这个项目中，我们将把新型Sers传感器集成到一个多细胞的活体气道模型中--我们的目标是监测对治疗的反应，以及在一系列基因型（和疾病表型）中以无与伦比的空间和时间分辨率进行修复的能力。成功的学生将在一个动态的多学科环境中工作，并接受拉曼光谱，微细加工，3D组织模型和细胞培养方面的培训。

3D tissue models derived from patient cells are increasingly important in developing new therapies. 3D models are crucial for the design of tissue-specific and patient-specific models of human diseases - animal models cannot embrace such a diversity of genotypes and do not always reflect how a human disease works. The non-invasive measurement of pH and redox potential using (Surface-Enhanced Raman Spectroscopy (SERS)), which reflect the metabolic status of cells and the capacity of tissues to repair and regenerate in 3D models, will enable effects of therapies to be monitored in a way that is not currently possible. In this project, we will integrate novel SERS sensors into a multicellular, live airway model - our goal is to monitor the response to therapy and the ability to repair with unparalleled spatial and temporal resolution across a range of genotypes (and disease phenotypes). The successful student will work in a dynamic multidisciplinary environment and receive training in Raman spectroscopy, microfabrication, 3D tissue models and cell culture.