Embryonic Development of the Cerebellum

_ROVIDED。 我们以前的工作集中在胚胎小脑颗粒细胞的规格。这里我们 我想把我们的注意力转向小脑的另一个主要神经元，浦肯野细胞和深部神经元。 神经元该项目的总体目标是确定四类地形的表达， 转录因子，LIM同源结构域基因（Lhxl，Lhx 3，Lhx 5和Lhx 9），碱性螺旋-环-螺旋基因 （Math 1，Mash 1，Ngn 1，Ngn 2），含有配对盒的基因（Pax 2）和偶跳基因（Evx 1，2）， 胚胎小脑原基中的浦肯野和深层神经元祖细胞群，并使用 这些标志物来检查塑造细胞命运规范的因素。局部感应因素的作用， 骨形态发生蛋白对基因表达和细胞特化模式的影响将在2010年的gone表达研究中进行测试。 鸡胚和德雷尔突变小鼠。 首先，我们将定义基因表达模式在泥和鸟类胚胎集中的时间段 当深层神经元和浦肯野细胞产生时。我们将通过研究BAC来进一步完善我们的分析 在另一个实施方案中产生了Lhxl、Lhx 3、Lhx 5、Lhx 9、Mathl、Mashl、Ngnl、Ngn 2和Evxl的转基因小鼠。 项目其次，我们将研究Dreher突变小鼠，因为它遭受BMPs的损失，并在卵内使用 在禽类胚胎中进行电穿孔以确定BMP 6的作用。转录因子中的BMP 7和GDF 7 表达模式最后，我们将研究这些转录因子在小脑细胞中的功能 通过分析具有靶向无效突变的小鼠和通过检测吗啉代反义寡核苷酸来区分 将寡核苷酸导入禽类胚胎。

_ROVIDED. Our previous work has focused on the specification of granule cells in the embryonic cerebellum. Here we wish to turn our attention to the other principal neuron of the cerebellum, the Purkinje cell and to the deep neurons. The overall aim of this project is to determine the topographic expression of four classes of transcription factors, LIM homeodomain genes (Lhxl, Lhx3, Lhx5, and Lhx9), basic helix-loop-helix genes (Math1, Mash1, Ngnl, Ngn2), Paired box containing genes (Pax2) and Even-Skipped genes (Evxl,2) in Purkinje and deep neuron progenitor cell populations in the embryonic cerebellar primordium, and to use these markers to examine factors that shape cell fate specification. The action of local inductive factors, BMPs on gene expression and cell specification patterning will be tested in gone expression studies in the chick embryo and in the Dreher mutant mouse. First, we will define patterns of gene expression in mudne and avian embryos focusing on the time periods when deep neurons and Purkinje cells are generated. We will further refine our analysis by studying BAC transgenic mice for Lhxl, Lhx3, Lhx5, Lhx9, Math1, Mash1, Ngnl, Ngn2 and Evxl generated in another project. Second, we will study the Dreher mutant mouse, because it suffers a loss of BMPs, and use in ovo electroporation in avian embryos to define the role of BMP6. BMP7 and GDF7 in transcription factor expression patterns. Finally, we will study the function of these transcription factors in cerebellar cell differentiation by analyzing mice with targeted null mutations and by transfecting morpholino antisense oligonucleotides into avian embryos.