Engineering synthetic post-transcriptional regulatory circuits in S. cerevisae for co-ordinated gene expression

该项目将探索开发用于合成生物学的新型转录后电路的选择。为了做到这一点，一系列的组件，包括RNA结合蛋白，RNA结合靶基序，降解决定子，这些组件的效应子和其他实体，将在不同的组合中进行研究，并在一系列新的情况下进行测试。将详细描述各种组件的特性，然后在新建电路的背景下研究它们的行为。其中一个探索的背景将是异源基因的调控表达，例如参与类菌孢素氨基酸（MAAs）生物合成的基因。此外，该项目将研究在合成电路中结合转录和转录后控制元件的好处。

This project will explore options to develop novel forms of posttranscriptional circuitry for use in synthetic biology. In order to do this, an array of components, including RNA-binding proteins, RNA binding target motifs, degrons, effectors of these components, and other entities, will be studied in different combinations and tested in a range of novel scenarios. The various components will be characterised in detail and then their behaviour will be studied in the context of newly-constructed circuits. One of the contexts explored will be that of regulated expression of heterologous genes, for example genes involved in the biosynthesis of mycosporin-like amino acids (MAAs).In addition, the project will examine the benefits of combining transcriptional and posttranscriptional control elements in synthetic circuitry.