Elucidating the role of a novel virus-induced nuclear condensate in non-coding RNA biogenesis and the importance of the royal family of m6A reader pro

非编码RNA（ncRNA）调节基因表达的能力为病毒调节病毒基因表达提供了独特的可能性。我们最近发现，疱疹病毒KSHV可以通过调节所谓的ncRNA调控网络中其他ncRNA物种的活性来操纵宿主细胞环状RNA的水平，以增强其自身的复制。然而，KSHV如何调节circRNA生物合成以在感染期间失调其水平尚不清楚。在这个项目中，我将研究（i）病毒诱导的核凝聚物在circRNA生物合成中的作用，以及（ii）宿主细胞m6A机制的作用，特别是新型m6A阅读器蛋白，病毒在感染过程中利用它来调节病毒和细胞的ncRNA和mRNA加工。因此，该项目旨在更好地了解ncRNA和m6A在病毒复制中的作用，以及在感染过程中如何转录后调节基因表达。

The ability of non-coding RNAs (ncRNAs) to regulate gene expression offers unique possibilities for viruses to modulate virus gene expression. We have recently shown that the herpesvirus, KSHV, can manipulate the levels of host cell circular RNAs to enhance its own replication, by regulating the activity of other ncRNA species in so-called ncRNA regulatory networks. However, how KSHV regulates circRNA biogenesis to dysregulate their levels during infection is unknown. In this project I will examine (i) the role of virus-induced nuclear condensates in circRNA biogenesis and (ii) the role of host cell m6A machinery, particularly novel m6A reader proteins, utilised by viruses to regulate both viral and cellular ncRNA and mRNA processing during infection. Therefore, the project aims to better understanding the roles of ncRNA and m6A in virus replication and how gene expression is regulated post-transcriptionally during infection.