Core--Functional Genomics

我们建议开发一种基础设施，允许对基因表达谱进行大规模分析。这些图谱将用于研究暴露于超级基金化学品及其代谢物的多种物种的毒理基因组反应。功能基因组学研究核心将为杜克大学SBRC申请中提出的大多数研究项目提供DNA微阵列分析的研究支持。FGRC的目标之一是通过其作为中心资源的功能促进学科间和学科内的研究。此外，通过进行独立的数据分析，这超出了任何单个研究项目的具体目标，我们将扩大SBRC的研究范围。这个核心的具体目标是：1.为DNA微阵列实验开发一个基础设施，以支持杜克大学的SBRC调查人员。这将包括就实验设计提供咨询，以及进行DNA微阵列处理、数据分析和数据存储。2.建立用于DNA微阵列基因表达数据分析的支撑结构。这将包括开发一个符合MIAME和MAML标准的数据库，用于存储和分析DNA微阵列 提供数据；提供基本的数据处理服务；为个别调查员提供资源，以便进行高级数据分析。3.促进杜克大学SBRC调查人员之间的互动。这将通过对来自单个SBRC项目的微阵列数据进行独立的基因组分析并将结果传达给所有调查人员来实现。我们建议通过识别和分析对所有项目中概述的研究特别感兴趣的基因来加强单个项目的微阵列研究，并将其作为促进SBRC研究人员之间合作的资源。

We propose to develop an infrastructure that will allow for the large-scale analysis of gene expression profiles. These profiles will be applied to studies of toxicogenomic responses from multiple species exposed to Superfund chemicals and their metabolites. The Functional Genomics Research Core will provide research support for DNA microarray analysis to the majority of the Research Projects proposed in the Duke SBRC application. One of the aims of the FGRC is to foster inter- and intra-disciplinary research though its function as a central resource. In addition, by performing independent data analyses, which are beyond the specific aims of any individual research project, we will expand the research scope of the SBRC. The specific aims of this Core are: 1. Develop an infrastructure for DNA microarray experimentation that will support SBRC investigators at Duke. This will included consultation on experimental design, and performing DNA microarray processing, data analysis and data storage. 2. Develop a support structure for the analysis of DNA microarray gene expression data. This will include developing a MIAME and MAML compliant database for the storage and analysis of DNA microarray data; providing basic data processing services; and providing resources for individual investigators to perform advanced data analysis. 3. Facilitate interactions among Duke University SBRC investigators. This will be accomplished by performing independent, genomic analysis of microarray data from individual SBRC projects and communicating results to all of the investigators. We propose to enhance microarrays studies from individual projects by identifying and analyzing genes that are of particular interest for the studies outlined in all of the projects, and serve as a resource to facilitate collaborations among SBRC investigators.