Tumor suppressor adenomatous polyposis coli and breast carcinogenesis

描述(申请人提供)：我们的总体目标是确定结肠腺瘤性息肉病(APC)基因在DNA损伤诱导的DNA修复活性和乳腺癌发生中的作用。APC表达下调是乳腺癌中的一种主要现象，但其在乳腺癌中的作用以及正常乳腺上皮细胞发生癌变的分子机制(S)尚不清楚。过去，突变的APC在各种癌症中的作用已经被研究过。在我们的研究中，我们提出了一个非常新颖的概念，即APC水平的下降是导致DNA修复能力下降的原因，这是正常乳腺上皮细胞癌变的细胞机制。我们的初步数据表明，APC蛋白与碱基切除修复蛋白相互作用，包括增殖细胞核抗原(PCNA)和脱嘌呤/脱嘧啶内切酶(APE)。我们推测，正常水平的APC促进与增殖细胞核抗原和APE形成活性复合体，该复合体通过碱基切除修复途径促进基本DNA的修复，从而防止正常乳腺上皮细胞中基因突变的积累。APC水平的降低会导致增殖细胞核抗原/APE复合体在基础DNA上的不适当结合，降低碱基切除修复活性，基因突变积累，以及正常乳腺上皮细胞的转化。为了验证这一假设，我们将使用经DNA损伤的乳腺癌致癌物处理的人类正常乳腺上皮细胞系。我们将：(1)确定致癌物暴露降低正常乳腺上皮细胞中APC基因的表达；(2)表征APC与增殖细胞核抗原和APE的结构与功能的关系；(3)利用具有功能纯化的修复复合体的新的碱基切除修复实验系统来表征这些复合体在致癌物处理的正常乳腺上皮细胞系中的动态变化；以及(4)检测APC作为基础DNA上的增殖细胞核抗原招募因子的功能，以调节致癌物处理和未处理的正常乳腺上皮细胞的碱基切除修复活性。该项目将首次为了解APC在DNA损伤诱导的碱基切除修复和正常乳腺上皮细胞转化中的分子基础提供一个新的范例，并将促进用于预防乳腺癌进展的化疗药物的开发。

DESCRIPTION (provided by applicant): Our overall goal is to determine the role of the adenomatous polyposis coli (APC) gene in DNA damage-induced DNA repair activity and breast carcinogenesis. Down-regulation of APC is a predominant phenomenon in breast cancer, but neither its role in this disease nor the molecular alteration(s) underlying carcinogenesis in normal breast epithelial cells have been characterized. In the past, the role of mutated APC has been investigated in various cancers. In our studies, we propose a highly novel concept that it is the decreased level of APC that is responsible for the decreased DNA repair capacity, which is the cellular mechanisms underlying carcinogenesis in normal breast epithelial cells. Our preliminary data indicate that APC protein interacts with base excision repair proteins, including proliferating cell nuclear antigen (PCNA) and apurinic/apydmidinic endonudease (APE). We hypothesize that the normal levels of APC promote formation of an active complex with PCNA and APE that facilitates repair of abasic DNA through a base excision repair pathway thereby preventing accumulation of gene mutations in normal breast epithelial cells. Decreased levels of APC result in inappropriate binding of the PCNA/APE-complex at the abasic DNA, decreased base excision repair activity, accumulation of gene mutations, and transformation of normal breast epithelial cells. To test this hypothesis, we will use human normal breast epithelial cell lines treated with DNA-damaging mammary carcinogens. We will: (1) Determine that the carcinogen exposure attenuates APC gene expression in normal breast epithelial ceils; (2) Characterize the structure-function relationships of APC with PCNA and APE; (3) Utilize a novel base excision repair assay system with functional purified repair complexes to characterize changes in the dynamics of these complexes in carcinogen-treated normal breast epithelial cell lines; and (4) Examine the function of APC as a PCNA recruiting factor onto abasic DNA to regulate base excision repair activity in carcinogen-treated and untreated normal breast epithelial cells. This project will, for the first time, provide a novel paradigm for understanding the molecular basis for APC function in DNA damage-induced base excision repair and the transformation of normal breast epithelial cells and will facilitate development of chemotherapeutics for prevention of breast cancer progression.