NCRR: UCSC Acquisition of a Thermo Electron LTQ-Mass Spectrometer

描述（由申请人提供）：十三UCSC教职员工走到一起，为购买最先进的Finnigan LTQ质谱仪系统（LTQ MS）开发此应用程序。该系统由高性能二维线性四极杆离子阱质谱仪（LTQ）组成。我们要求NCRR资助购买该系统，以大大扩展和现代化我们的质谱MS设施，该设施致力于UCSC化学和生物化学（CBC），分子，细胞和发育生物学（MCD）和环境毒理学（ETOX）部门的研究。共享MS设施目前拥有两台质谱仪，而另外两台质谱仪则位于各个实验室中，可应要求提供给生物医学研究人员。虽然这些工具得到了很好的维护，但每个工具都有限制，削弱了其支持本可以在这所大学进行的研究的质量和广度的能力。如果没有额外的，更新的设备，我们为我们的教师提供一流的研究基础设施的能力将继续受到影响。这个问题只会在未来变得更加严重，因为这四个部门的生物医学研究人员预计将在未来十年内扩大50%。收购LTQ MS将在缓解这些问题方面发挥重要作用。10个主要用户的研究倡议可分为两大领域。每一个都对基础生物科学以及相关的生物技术应用和疾病治疗产生深远的影响。广泛的主题领域包括：1）基于ESI-Linear Ion-Trap的蛋白质组学研究复杂的生化过程2）ESI-Linear Ion-Trap方法来理解信号转导途径。区域1的五个主要用户（Fink、Holman、Lokey、Millhauser和Smith）在依赖于拟议仪器的分辨率和灵敏度的项目中使用蛋白质组学方法。与区域2相关的其他五个主要用户（Hartzog、凯洛格、Noller、Ottemann和Tamkun）利用该技术进行生物化学细胞机器表征。三名未成年用户将使用该仪器研究蛋白质结构和功能，并阐明涉及毒理学的细胞机制。

DESCRIPTION (provided by applicant): Thirteen UCSC faculty members have come together to develop this application for the purchase of a state-of-the-art Finnigan LTQ Mass Spectrometer System (LTQ MS). This system consists of a high- performance 2D linear quadrupole ion trap mass spectrometer (LTQ). We are asking the NCRR to fund the purchase of this system to greatly expand and modernize our Mass Spectrometry MS Facility, which is dedicated to research in UCSC's Departments of Chemistry and Biochemistry (CBC), Molecular, Cell, and Developmental Biology (MCD), and Environmental Toxicology (ETOX). The shared MS Facility currently houses two mass spectrometers, while 2 additional mass spectrometers are housed in individual labs and available on request to the biomedical research faculty. While these instruments are well maintained, each has limitations that undercut its ability to support the quality and breadth of research that could otherwise be undertaken at this university. Without additional, updated equipment, our ability to provide our faculty with a first class research infrastructure will remain compromised. This problem will only become exacerbated in the future, as the biomedical research faculty of these four departments is projected to expand by 50% over the next decade. Acquisition of the LTQ MS will play a large role in alleviating these problems. The research initiatives of the ten major users can be divided into two broad areas. Each has far reaching implications for basic biological science, as well as for related biotechnology applications and the treatment of disease. The broad subject areas include: 1) ESI-Linear Ion-Trap based proteomics to study complex biochemical processes 2) ESI-Linear Ion-Trap approaches to understanding signal transduction pathways. The five major users in Area 1 (Fink, Holman, Lokey, Millhauser, and Smith) use proteomic methods on projects that depend on the resolution and sensitivity of the proposed instrument. Five other major users associated with Area 2 (Hartzog, Kellogg, Noller, Ottemann, and Tamkun) utilize this technology for biochemical cellular machinery characterization. Three Minor Users who will employ the instrument to investigate protein structure and function, and to clarify cellular mechanisms involved toxicology.