TWO-PHOTON MICROSCOPE SYSTEM FOR IN-VIVO IMAGING: CANCER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：双光子显微镜允许可视化细胞过程，如细胞迁移、细胞到细胞和细胞到基质的相互作用、肿瘤的生长、微生物感染以及活动物完整器官的三维自然环境中的基因表达。在过去的几年里，双光子显微镜彻底改变了我们对发育、体内平衡和病理过程的看法。我们建议在Wistar研究所建立一个活体双光子显微镜系统，通过获得徕卡TCS SP2光谱共焦和双光子显微镜，并结合光谱物理宽带MAI-TAI飞秒脉冲激光器。拟议仪器的主要用户将是一组来自广泛学科的Wistar调查人员，包括免疫学、肿瘤生物学、皮肤生物学和传染病研究。需要将实验数据从培养模型转化为活动物，并将动态细胞过程的静态信息扩展到体内的四维动态分析，这极大地增加了维斯塔尔研究所对活体成像的需求。这样的研究不能用现有的设备进行。在前景看好的初步研究的基础上，研究人员将深入探讨体内免疫系统和肿瘤细胞之间的相互作用，炎症和感染性疾病期间的免疫细胞相互作用，以及肿瘤发生和发展过程中的黑素细胞/黑色素瘤细胞行为。仪器将设在研究所的中央位置，以便所有用户都能方便地进入，由专门的仪器操作员管理，并由显微镜咨询委员会监督。来自不同领域的研究人员将有新的互动和发起跨学科研究项目的机会，这将大大推进他们由NIH资助的项目。预计该仪器将有助于对疾病的病理机制获得新的见解，包括自身免疫、癌症和慢性感染。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Two-photon microscopy allows for the visualization of cellular processes, such as cell migration, cell-to-cell and cell-to-matrix interactions, growth of tumors, microbial infection, and gene expression within the three- dimensional, natural environment of intact organs in living animals. During the past few years two-photon microscopy has revolutionized our view of developmental, homeostatic, and pathologic processes. We propose to establish an intravital two-photon microscopy system at The Wistar Institute through the acquisition of a Leica TCS SP2 Spectral Confocal and Two-Photon Microscope in combination with a Spectra-Physics Broadband Mai-Tai femtosecond-pulsed Laser. The major users of the proposed instrument will be a group of Wistar Investigators drawn from a wide range of disciplines, including immunology, tumor biology, skin biology, and infectious disease research. The need to translate experimental data from culture models into living animals, and to expand static information of dynamic cellular processes into four- dimensional kinetic .analyses in vivo has greatly augmented the need for intravital imaging at the Wistar Institute. Such research cannot be conducted by equipment currently available. Based on promising preliminary studies, the Investigators will explore in-depth the interactions between the immune system and tumor cells in vivo, immune cell interactions during inflammatory and infectious diseases, and melanocyte/melanoma cell behavior during tumor development and progression. The instrument will be centrally located at the Institute to enable convenient access for all users, will be managed by a dedicated instrument operator, and supervised by the Microscope Advisory Committee. Investigators from diverse fields will have new opportunities for interaction and the initiation of interdisciplinary research projects, which will significantly advance their NIH-funded projects. It is anticipated that the instrument will help to obtain novel insights into the pathomechanisms of disease, including autoimmunity, cancer, and chronic infections.