Advantages of HPLC with Ultra Stable Phases

描述（申请人提供）：之前，我们展示了如何制作和使用稳定的氧化锆RPLC介质，介绍了两种新技术，热调谐串联柱（T3C）色谱和高温超快速液相色谱（HTUFLC）。我们现在提议制造和表征一些创新的高度稳定的二氧化硅（SiO2）相，这些相将使用局限于二氧化硅表面单层的超交联表面聚合物网络。然后，我们将这些材料应用于药物，环境和工作场所毒物的高速分析，并最终与大卫·马迪曼教授合作，用于复杂的生物样品。我们的目标是探索新的衍生化技术的各个方面，包括传统的LC，填充毛细管LC和毛细管电色谱。我们将探讨高稳定相的可用性使HPLC中的两个一般概念成为可能。首先，我们提出基于相的快速、全面的二维高效液相色谱（2D-LC）。由于常规支架的热限制，在HPLC中使用高温的优势很少被探索。特别是，当温度升高到200℃时，由此产生的粘度降低20倍，分析速度提高近20倍。我们将利用HTUFLC的高速度来提高2D-LC的速度，使其成为更实用的常规方法。使用较高的温度也有利于使用较长的色谱柱，特别是在毛细管格式中，特别是用于微检测。其次，我们建议将等压T3C概念扩展到梯度洗脱色谱。这两种方法都是基于高热稳定性相的可用性。

DESCRIPTION (provided by applicant): Previously, we showed how to make and use stable zirconia RPLC media, introducing two new techniques, thermally tuned tandem column (T3C) chromatography and high temperature ultra-fast liquid chromatography (HTUFLC). We now propose to make and characterize a number of innovative highly stable silica (SiO2) phases that will use hypercrosslinked surface polymer networks confined to a monolayer on the silica surface. We will then apply these materials to the high-speed analysis of pharmaceuticals, environmental and workplace toxicants, and ultimately, in collaboration with Professor David Muddiman, to complex biological samples. Our goal is to explore all aspects of the new derivatization technology, including conventional LC, packed capillary LC and capillary electrochromatography. We will explore two general concepts in HPLC made possible by the availability of high stability phases. First, we propose to develop fast, comprehensive two-dimensional HPLC (2D-LC) based on our phases. Due to the thermal limitations of conventional supports, the advantages of using high temperatures in HPLC have hardly been explored. In particular, upon increasing the temperature to 200 degrees C, the resulting 20-fold decrease in viscosity gives a nearly 20-fold increase in analysis speed. We will use the high speed of HTUFLC to increase the speed of 2D-LC and make it a much more practical routine method. The use of higher temperatures also facilitates the use of longer columns, especially in a capillary format, particularly for microdetection. Second, we propose to extend the isocratic T3C concept to gradient elution chromatography. Both approaches are predicated on the availability of phases of high thermal stability.