Understanding the role of non-coding RNAs in intercellular communication and how its potential for the detection and inhibition of pre-metastatic canc

博士学位建立在小组在确定细胞外囊泡影响癌症向骨转移的机制方面的成功工作的基础上。细胞外囊泡（EVs）曾经被认为只不过是细胞排出废物的一种方式，现在被认为是细胞分泌RNA、DNA和蛋白质的一种蓄意方式，以重塑组织并充当信号载体。这些有些被忽视的细胞器可能是理解我们身体组织和系统如何沟通的关键，特别是在复杂疾病（如癌症和功能失调的免疫细胞）中。该项目将探索这些ev的非编码RNA元件，以确定它们控制的潜在调控机制。该项目将结合数据分析，建立由这些非编码rna组改变的信号和调节机制类型的实验假设，并结合分子生物学在前列腺癌的体外模型中验证这些假设。该项目将需要分子生物学技术的发展，以及分析大型数据集和基于现有数据源创建实验假设的能力。这些技能提供了优秀的多学科基础，适合bbsrc的职权范围内的方法。

The PhD builds upon the successful work of the group in identifying the mechanisms by which extracellular vesicles influence the metastasis of cancer to bone. Once thought to be little more than a way for cells to offload waste, extracellular vesicles (EVs) are now recognised to be a deliberate way for a cell to secrete cargos of RNA, DNA and proteins to reshape tissues and act as signal carriers. These somewhat overlooked organelles may hold the key tounderstanding how tissues and systems in our bodies communicate, particularly within complex diseases (e.g., cancer and dysfunctional immune cells).The project will explore the non-coding RNA elements of these EVs to determine the potential regulatory mechanisms they control. The project will be a mixture of data analysis to build experimental hypothesises of the types of signalling and regulatory mechanisms that are altered by these groups of non-coding RNAs, combined with molecular biology to validate these hypotheses within in vitro models of prostate cancer.The project will require the development of molecular biology techniques alongside the ability to analyse large datasets and create experimental hypothesise based on existing data sources. These skills provide an excellent multidisciplinary basis and fit within the BBSRCs remit of approaches.