Egg to Embryo: Gene Regulatory Circuitry in Development

本更新计划项目申请的主要目标仍如其 前，以确定“调控信息流的分子基础，在早期胚胎 发展“然而，在很大程度上，由于我们在这一方案上的工作， 这些词的含义已经改变，这个更新申请的重点是 我们现在面临的一个机会是解决胚胎中调节相互作用流的问题 通过确定指导其发育的基因调控网络（GRN）。GRN主要由基因组顺式调控序列控制编码转录因子和一些信号组分的基因所规定的相互作用组成。初始输入是母体因素，每个空间域的最终输出是编码分化蛋白的基因的激活。在戴维森组件的程序，GRN分析的胚胎发生在海胆，球海胆purpuratus将扩展到整个胚胎，几乎整个胚胎发生（从受精后不久到约2.5天）。弗雷泽组件专注于使用海胆和海鞘胚胎成像GRNs活动的新方法。在莱文组件的关键联系的内中胚层GRN的海胆胚胎将寻求在海鞘胚胎，并将检查的可能性，有一个基础的顺式调节代码，这一基本过程中的胚胎发生之间的脊索动物和棘皮动物共享。行政科将监督管理、预算和实验室间有关财务、材料和知识事项的协调。还要求建立一个与现有设施类似但不完全相同的研究核心服务设施，其基本作用是提供S. Purpuratus的三个研究组成部分。

The major objective of this Renewal Program Project Application remains as stated in its predecessor, to determine "the molecular basis of regulatory information flow in early embryonic development." However, to a significant extent as a result of our work on this Program, the meaning of these words has been transformed, and this Renewal Application is focused on the opportunity now confronting us to solve the problem of regulatory interaction flow in the embryo by determining the gene regulatory network (GRN) that directs its development. The GRN consists largely of interactions mandated by genomic cis-regulatory sequences controlling genes that encode transcription factors and some signaling components. The initial inputs are maternal factors, and the terminal output in each spatial domain is activation of genes encoding differentiation proteins. In the Davidson Component of the Program, GRN analysis of embryogenesis in the sea urchin, Strongylocentrotus purpuratus will be extended to the whole of the embryo, and almost the whole of embryogenesis (from shortly after fertilization to about 2.5 days). The Fraser Component is focused on novel approaches to imaging the activity of GRNs, using sea urchin and ascidian embryos. In the Levine Component key linkages of the endomesoderm GRN of the sea urchin embryo will be sought in the ascidian embryo, and the possibility will be examined that there is a basal cis-regulatory code for this fundamental process in embryogenesis shared between chordates and echinoderms. The Administrative section will oversee management, budget, and interlaboratory coordination with respect to financial, material and intellectual matters. A Research Core Service Facility similar but not identical to the current one is also requested, the basic role of which is to provide embryos, eggs and nuclear extracts of S. purpuratus to the three Research Components.