Identification of new plant defense signaling pathways

描述(申请人提供)：与哺乳动物的几种细菌病原体一样，许多植物病原菌将蛋白质注入宿主细胞细胞质。植物对细菌侵袭的识别导致至少三条防御信号通路介导的防御反应。为了测试是否存在其他植物防御信号通路，我将识别参与这些通路的基因。我将使用全基因组微阵列分析来鉴定拟南芥基因，这些基因是对细菌病原体丁香假单胞菌诱导的，但不依赖于涉及水杨酸、茉莉酸和乙烯的典型防御信号通路而被触发。我将对这些基因中的192个进行突变分析，以确定那些可能参与植物抵御细菌病原体所需的新的疾病信号途径的基因。我将研究这些途径在植物抵御病原体方面的作用，并采用不同的定殖策略。此外，我将详细说明这些路径是如何触发的，它们是如何发挥作用的，以及它们是如何组织的。由于这些研究将确定抗病所需的新的植物信号通路，因此它们对于研究植物对微生物攻击的免疫反应至关重要。

DESCRIPTION (provided by applicant): As is the case with several bacterial pathogens of mammals, many plant pathogenic bacteria inject proteins into the host cell cytoplasm. Plant recognition of bacterial attack results in a defense response mediated by at least three defense signaling pathways. To test whether additional plant defense signaling pathways exist, I will identify genes that participate in these pathways. I will use whole genome microarray analysis to identify Arabidopsis thaliana genes that are induced in response to the bacterial pathogen Pseudomonas syringae but are triggered independently of the canonical defense signaling pathways involving salicylic acid, jasmonic acid and ethylene. I will perform mutational analysis of 192 of these genes to identify those genes that are likely to participate in novel disease signaling pathways that are required for plant defense against bacterial pathogens. I will study the role of these pathways in plant protection against pathogens with diverse colonization strategies. Further, I will detail how these pathways are triggered, how they function, and how they are organized. As these studies will identify novel plant signaling pathways that are required for disease resistance, they are crucial to the study of the plant immune response to microbial attack.