The impact of skincare ingredients on skin-resident immune cell responses

皮肤作为屏障、感觉和免疫器官，由对宿主防御和组织内环境稳定至关重要的免疫常驻细胞组成的复杂网络组成。本项目旨在研究T细胞、肥大细胞和巨噬细胞等关键皮肤常驻免疫细胞对炎性挑战的反应，并评估护肤成分对这些反应的影响。体外细胞系统将被用来确定不同炎症刺激下免疫细胞活性的关键标志，并了解不同护肤成分(如视黄醇、烟酰胺)治疗如何影响免疫细胞表型和对细胞增殖、激活和介质分泌的影响。此外，体外人类皮肤移植将被用来研究皮肤微环境对免疫细胞活性的影响。该项目将利用RNA测序、流式细胞术和高通量组织学技术来了解化妆品成分如何针对细胞炎症途径，并设计特定护肤品的抗炎潜力的预测模型。因此，该项目承诺在皮肤研究的实验室和临床方面提供深入的经验，生物信息学培训，并有机会获得对护肤配方和科学研究的商业应用的见解。

The skin serves as a barrier, sensory, and immune organ and consists of a complex network of immune resident cells crucial for host defense and tissue homeostasis.This project aims to investigate the response of key skin resident immune cells e.g. T cells, mast cells and macrophages, to inflammatory challenges and assess the impact of skin care ingredients on these responses. In vitro cell systems will be used to define key markers of immune cell activity following different inflammatory stimuli and to understand how treatment with different skin care ingredients (e.g. retinol, nicotinamide) may influence the immune cell phenotype and have an impact cell proliferation, activation and mediator secretion.Furthermore, ex vivo human skin explants will be used to investigate the impact of the skin microenvironment on immune cell activities. RNA sequencing, flow cytometry and high throughput histology techniques will be utilised to understand how cosmetic ingredients might target cellular inflammatory pathways and to design predictive models of the anti-inflammatory potential of specific skin care products.Thus, this project promises to deliver an in-depth experience in both laboratory and clinical aspects of skin research, bioinformatics training, and the opportunity to gain insights into skin care formulation and commercial applications of scientific research.