Electrophysiology/Cell Culture Core

电生理学/细胞培养核心旨在为PPG研究人员提供一系列常见的 利用培养的人肠上皮细胞系(Caco-2、T84、HT-29和NCM460)，获得 在培养上皮片和天然组织上进行大量电生理测量。两者都有 核心组件包含最先进的设备，由具有完整技术专业知识的人员运行，因此 培训、协助实验设计、数据收集、数据分析和解释 如果是这样的话。这个核心是由盖尔·赫克特博士和穆里纳里尼·拉奥博士双重指导的。他们都有广泛的 培养的肠上皮细胞、天然肠上皮细胞各方面的电生理测量经验 组织和原代肠道细胞。具体目标是为调查人员和项目提供这一 培养的人肠上皮细胞包括Caco-2、T84、HT-29和NCM460的计划项目 生长在塑料或可渗透(Transwell)过滤器上.在Ussing小室中进行电生理测量 包括短路电流(ISC)、跨上皮电阻(TER)、离子通量分析 培养的肠片和天然肠道组织；以及作为 评估离子传输的补充方法。为电生理提供这些细胞 测量将促进和加速所有计划调查人员和 将使这些细胞的生长条件标准化，从而允许数据的直接关联 为每个项目获得的。

Electrophysiology/Cell Culture Core is designed to provide PPG investigators with an array of commonly utilized cultured human intestinal epithelial cell lines (Caco-2, T84, HT-29, and NCM460) and access to a multitude of electrophysiological measurements on both cultured epithelial sheets and native tissues. Both Core components house state-of-the-art equipment, which is run by personnel with full technical expertise so that training, assistance with experimental design, data collection, data analysis and interpretation are provided. This Core is under dual direction by Drs. Gail Hecht and Mrinalini Rao. They both have extensive experience in all aspects of electrophysiological measurements in cultured intestinal epithelia, native intestinal tissue, and primary intestinal cells. The Specific Aims are to provide the investigators and projects within this Program Project with human cultured intestinal epithelial cells including Caco-2, T84, HT-29, and NCM460 grown on plastic or permeable (Transwell) filters; electrophysiological measurements in Ussing chambers including short circuit current (Isc), transepithelial electrical resistance (TER), ion flux analysis, on both cultured intestinal sheets and native intestinal tissues; and spectrofluorometric measurements as a complementary approach to assessing ion transport. The provision of these cells for electrophysiologic measurements will facilitate and expedite the proposed experiments of all of the Program investigators and will standardize the conditions under which these cells are grown thus allowing for direct correlation of data obtained for each of the Projects.