Cell Imaging

细胞和组织成像核心旨在为该计划中的研究人员和项目提供支持 项目获得最先进的技术和设备以及充分利用这些技术和设备的专业知识 能力的因此，除了提供设备外，所提供的服务还包括技术支助， 培训，并协助实验设计，数据收集，数据分析，解释， 准备演示质量图像。核心由Jerrold Turner博士管理， 一位胃肠外科病理学家，他在转染细胞的产生方面也有丰富的经验 线，定量和定性荧光成像方法，和免疫染色技术， 位于特纳博士的办公室和实验室附近。经验丰富的专业技术人员将 支持核心数据库的运作，并协助调查员处理核心数据库利用的所有方面。具体 细胞和组织成像核心的目的是为该计划内的研究者和项目提供 项目1.产生表达荧光融合蛋白的细胞系，包括构建 合适的表达构建体和细胞系的转染、分选和维持，2.时间间隔 活细胞单层的成像与同时电生理测量，3.荧光 恢复后光漂白（FRAP）的研究，4。培养细胞和冷冻细胞的免疫荧光 组织切片，5.采集后处理，包括定量分析和反褶积，6. 来自实验动物的组织的组织学制备和分析，以及7.免疫酶 动物组织染色。细胞和组织成像核心设施包括两个落射荧光光谱仪， 带加热/冷却载物台、z电机、自动电子滤光轮和冷却CCD的显微镜 相机另外还提供一台配备高分辨率彩色数码相机的双头光学显微镜。 在核心内有多个离线数据分析工作站。细胞和组织成像核心是 由芝加哥大学的其他核心设施支持，包括2个共聚焦显微镜和2个 集成显微镜核心中的倒置荧光显微镜和高速细胞分选 流式细胞术核心的一部分 概要：该核心为项目提供常规和复杂的显微镜方法 Investigators.这些方法既需要设备，也需要技术技能，而这些通常是不具备的 个别实验室。他们对计划项目研究者的可用性将使其能够完成 研究，否则无法完成，并将提高效率和成本效益， 其他研究。因此，这一核心对于整个计划项目的成功至关重要。

The Cell and Tissue Imaging Core is designed to provide the investigators and projects within this Program Project access to state-of-the-art techniques and equipment and the expertise to use these to their fullest capabilities. Thus, in addition to provision of equipment, services offered will include technical support, training, and assistance with experimental design, data collection, data analysis, interpretation, and preparation of presentation quality images. The core is managed by Dr. Jerrold Turner, a practicing gastrointestinal surgical pathologist who also has extensive experience with generation of transfected cell lines, quantitative and qualitative fluorescent imaging approaches, and immunostaining techniques, and is located adjacent to Dr. Turner's office and laboratory space. An experienced dedicated technician will support the operation of the core and assist investigators with all aspects of core utilization. The Specific Aims of the Cell and Tissue Imaging Core are to provide the investigators and projects within this Program Project with 1. creation of cell lines expressing fluorescent fusion proteins, including construction of appropriate expression constructs and transfection, sorting, and maintenance of cell lines, 2. time lapse imaging of live cell monolayers with simultaneous electrophysiological measurement, 3. fluorescence recovery after photobleaching (FRAP) studies, 4. immunofluorescence of cultured cells and frozen tissue sections, 5. post acquisition processing, including quantitative analysis and deconvolution, 6. histological preparation and analysis of tissues from experimental animals, and 7. immunoperoxidase staining of animal tissues. The Cell and Tissue Imaging Core facilities include two epifluorescence microscopes with heated/cooled stages, z-motors, automated electronic filter wheels, and cooled CCD cameras. A separate two-headed light microscope with high resolution color digital camera is also available. Multiple offline data analysis workstations are available within the core. The Cell and Tissue Imaging Core is supported by other core facilities at The University of Chicago, including 2 confocal microscopes and 2 inverted epifluorescence microscopes within The Integrated Microscopy Core and high speed cell sorting within the Flow Cytometry Core. SUMMARY: This core provides both routine and sophisticated microscopy approaches to Program Project Investigators. These approaches require both equipment and technical skills that are not typically available to individual laboratories. Their availability to Program Project Investigators will make it possible to complete studies that could not otherwise be accomplished and will increase both efficiency and cost-effectiveness of other studies. Thus, this core is vital to the success of the overall Program Project.