STURCTURE/PROPERTIES OF ALTERED FORMS OF DENTIN AND CEMENTUM

该项目的目标是充分表征牙科硬组织，提供有关其生物力学和矿化的重要信息，并对其进行修饰以增强其聚合物基粘接剂的粘附性。这项修订后的提案是一项为期11-15年的计划项目拨款、牙科硬组织的特征和修改。近十年来，人们对牙本质基质的性质有了新的了解，包括它的结构、机械性能和酸蚀特性，以及它与牙本质聚合物粘结剂的界面。在这个拟议的时期，基质将扩大到包括其他形式的牙本质，以及牙釉质和牙骨质。该计划项目有一个实验室、统计和行政核心和4个项目：改变形式的牙本质和牙骨质的结构/特性；牙本质的生物力学；与牙齿钙化组织的粘结区；以及组织工程牙齿结构的工具。中心将对所有项目进行行政协调，为所有项目的所有实验收集牙齿和提供样本，管理数据库，进行统计分析，并确保传播结果。项目8将确定关键的材料结构/性能关系，从而能够定量和唯一地确定各种形式的牙本质、其因年龄、疾病过程以及治疗和牙骨质而引起的变化。这一新知识将为更好地了解这些组织及其变异奠定基础，并提供对牙科钙化组织的洞察，这将有助于推进组织工程的概念。这些信息还将被用来对牙齿的机械性能进行建模，包括它的基本弹性常数以及在一个项目中导致抗断裂和失效的因素。这些模型将在三个长度尺度上制作，从组成到连续。因此，项目8和9在理解牙齿钙化组织方面是相辅相成的。项目10将利用项目8和项目9的信息来开发新的基材形态和性能，以增强与树脂基复合材料的粘结耐久性。项目11将使用与其他项目相同的方法来评估用于牙齿组织工程的转基因小鼠模型。它将重点分析小鼠模型中的牙本质和牙骨质，并作为一种工具来确定转化生长因子-β在这些组织的发育和修复中的作用。

The goal of this program project is to fully characterize dental hard tissues, provide important information on their biomechanics and mineralization, and modify them to enhance adhesion of polymer based adhesives of them. This revised proposal is for years 11-15 of a program project grant, Characterization and Modification of Dental Hard Tissues. For ten years new information has been generated about the nature of the dentin substrate for preventive and restorative dentistry, including its structure, mechanical and etching properties, and its interface with polymeric dentin adhesives. In this proposed period the substrate will be expanded to include other forms of dentin, as well as enamel and cementum. The program project has a Laboratory, Statistical and Administrative Core and 4 projects: Structure/Properties of Altered Forms of Dentin and Cementum; Biomechanics of Dentin; The Bonding Zone with Dental Calcified Tissues; and Tools for Tissue Engineering Tooth Structure. The Core will coordinate all projects administratively, collect teeth and provide specimens for all experiments on all projects, manage the data base, conduct statistical analyses and insure that the results are disseminated. Project 8 will identify key materials structure/properties relationships that allow quantitative and unique identification of a broad array of various forms of dentin, its alterations due to age, disease processes and treatment and cementum. The new knowledge will serve as a foundation for improved understanding of these tissues and their variations as well as provide insights into dental calcified tissues that will be useful to advancing concepts is tissue engineering. The information also will be used to model mechanical properties of the tooth, including its fundamental elastic constants and factors contributing to fracture resistance and failure in one project. These models will be made on 3 length scales, from the constituent to the continuum. Thus projects 8 and 9 are complementary to each other in understanding dental calcified tissues. Project 10 will use the information from projects 8 and 9 to develop novel substrate morphologies and properties that will enhance the durability of bonds to resin based composites. Project 11 will use the same methods as the other projects to evaluate transgenic mouse models for tissue engineering of teeth. It will focus on the analysis of dentin and cementum in the mouse model, and be used as a tool to determine the role of TGF-Beta on the development and repair of these tissues.