CORE--Crystallographic data collection at the APS

这个核心项目的目的是在阿贡国家实验室高级光子源工业高分子晶体协会协作访问团队(IMCA-CAT)的设施中有效和高效地获取和处理衍射数据。在解决真核蛋白质中多个剪接位点的结构和识别结构含义的过程中，结晶学数据收集不应该成为瓶颈。数据收集不会成为瓶颈，只要：(A)收集常规数据的程序变得高效和可靠；(B)为多波长数据收集选择峰值和拐点能量的程序稳定；(C)可以获得非标准但有用的多波长或优化异常能量；以及(D)处理衍射数据的程序易于使用，并能跟上数据采集速度。在IMCA-CAT设施中，这些任务在很大程度上已经完成，达到了本轮结构基因组学项目所用的生产能力水平。随着需求在不久的将来增加，我们正在采取行动，从所有四个方面改善设施，以满足这一需求。具体地说，我们打算在IMCA-CAT的插入装置光束线17-ID上安装一个样品装载机器人系统，并对该光束线上的单色仪、可视化光学系统和准直组件进行改进。在IMCA-CAT的弯曲磁铁光束线17-BM上，我们计划安装一个准直镜，应该会在信噪比上提供两倍以上的改善。随着多重拼接项目的进行，我们将提供一个有效的数据采集和处理场地，并稳步改善设施。多重拼接项目和IMCA-CAT的主要药物用户群体将从彼此的进步中受益。

The aim of this core project is to acquire and process diffraction data effectively and efficiently at the facilities of the Industrial Macromolecular Crystallography Association Collaborative Access Team (IMCA-CAT) at the Advanced Photon Source, Argonne National Laboratry. Crystallographic data collection should not be a bottleneck in the process of solving structures and identifying the structural implications of multiple splicing sites in eukaryotic proteins. Data collection will not become a bottleneck as long as (a) procedures for collecting routine data are rendered efficient and fool-proof; (b) procedures for choosing peak and inflection-point energies for multiwavelength data collection are stable; (c) access to non-standard but useful multiwavelength or optimized-anomalous energies is available; and (d) procedures for processing the diffraction data are easy to use and can keep up with the data acquisition rate. At the IMCA-CAT facilities, these tasks are for the most part already accomplished for the level of throughput used in the current round of structural genomics projects. As the demand increases in the near future, we are moving to improve the facilities in all four ways in order to address that demand. Specifically, we intend to install a sample-loading robotics system on IMCA-CAT's insertion-device beamline, 17-ID, and make improvements in the monochromator, the visualization optics, and the collimation assembly on that beamline. On IMCA-CAT's bending-magnet beamline, 17-BM, we plan the installation of a collimating mirror that should provide a better than twofold improvement in signal-to-noise ratios. As the multiple-splicing project proceeds,we will provide a site for effective data acquisition and processing, and make steady improvements in the facilities. The multiple-splicing project and IMCA-CAT's primary constituency of pharmaceutical users will benefit from one another's advancements.