Core--IMMUNODEFICIENT MOUSE COLONY

免疫缺陷小鼠核心设施的目的是为该项目的研究人员提供生物模型，以在体内测试前列腺癌的致瘤性。严重联合免疫缺陷（SCID）小鼠人肿瘤异种移植瘤模型将是这些研究中主要采用的模型，但其他动物模型如胸腺裸鼠模型也可能被采用。SCID小鼠异种移植模型检测肿瘤生长、粘附性、渗透、局部扩散和远处转移。具体的可测量终点是定性的和定量的。定性评估包括大体解剖，以及人类前列腺异种移植的免疫形貌的组织学和表型描述。定量评估包括肿瘤移植物的大小、数量、浸润深度和动物存活数据。利用异位和正位注射人类前列腺细胞系建立了模型。

The purpose of the immunodeficient mouse core facility is to serve the investigators in this program project by providing them with access to biological models to test the tumorigenicity of prostate carcinoma in vivo. The severe combined immune-defcient (SCID) mouse human tumor xenograft model will be the predominant model employed in these studies but other animal models such as the athymic nude mouse model may also be utilized. The SCID mouse xenograft models test tumor growth, adhesiveness, penetration, local spread and distant metastases. The specific measurable end points are both qualitative and quantitative. Qualitative assessments include gross anatomic as well as histologic and phenotypic descriptions of the immunotopography of human prostate xenografts. Quantitative assessment includes tumor xenograft size, number, depth of invasion, and animal survival data. Models have been developed utilizing both ectopic as well as orthotopic injections of human prostate cell lines, This core will support the projects in the following manner: 1) Provide the technical expertise to perform the SCID mouse-human tumor xenograft experiments. 2) Assist with the design and interpretation of experiments utilizing SCID mouse models of human prostate tumor growth and metastasis. 3) Provide fixed, frozen, and fresh tissue from the SCID mouse human xenografts for analysis as described in projects 0001, 0002 and 0005.