CORE--Gene Microarray

基因微阵列共享资源（GMSR）是一个全方位服务的DMA微阵列设施提供OHSU癌症研究所（CI）研究人员以合理的成本高品质的表达谱分析服务。核心设施有一名全职博士生主任，一名实验室经理，两名研究技术人员和一名信息学家。服务包括：1）实验设计和样品制备方面的咨询; 2）基因芯片寡核苷酸阵列的表达谱分析; 3）数据管理和存储; 4）使用配备高性能PC和微阵列分析软件的信息学实验室; 5）支持微阵列数据分析; 6）微阵列技术、数据格式和数据分析软件方面的教育和培训。数据管理系统通过信息学和生物统计学共享资源进行维护，高级数据分析也可通过此 姐妹Cl核心。我们已经培养了一批具有基本能力、过去在微阵列技术方面的经验和复杂性的专业人员和研究人员，以确保适当的实验设计和解释。我们完善的基础设施和质量控制程序支持向CI研究人员提供快速，可重复和准确的微阵列数据。在过去的三年里，GMSR已经为Cl成员处理了50多个基于Affytron阵列的项目，吞吐量超过600个阵列。自五年前开发以来，CI成员也可以通过这种共享资源获得斑点微阵列服务。

The Gene Microarray Shared Resource (GMSR) is a full service DMA microarray facility providing OHSU Cancer Institute (Cl) investigators with high-quality expression profiling services at reasonable cost. The core facility has a full-time PhD director, a laboratory manager, two research technicians and an informaticist. Services include: 1) consultation on experiment design and sample preparation; 2) expression profiling on GeneChip oligonucleotide arrays; 3) data management and storage; 4) access to an informatics laboratory equipped with high-performance PCs and microarray analysis software; 5) support for microarray data analysis; and 6) education and training on microarray technology, data formats, and data analysis software. Data management systems are maintained through the Informatics and the Biostatistics Shared Resources, and advanced data analysis is also available through this sister Cl core. We have developed a critical mass of professionals and investigators with essential capabilities, past experience in microarray technology, and sophistication to assure appropriate design and interpretation of experiments. Our well-established infrastructure and quality-controlled procedures support the delivery of rapid, reproducible and accurate microarray data to Cl investigators. In the past three years, the GMSR has processed over 50 Affymetrix array-based projects for Cl members with a throughput of over 600 arrays. Cl members have also had access to spotted microarray services through this shared resource since its development five years ago.