Molecular Genetics of the MHC

桑格中心在过去的一个财政年度完成了对来自六个个体的HLA III类区域的45 kb片段的测序，其中从每个PCR片段创建了微型文库，并对多个克隆进行测序以创建重叠群。桑格中心没有解决的短片段的进一步测序正在我的实验室完成。与此同时，来自Bob Stephens博士小组的Beena Neelam已经开始从桑格中心提供的单个克隆序列中组装基因组序列的重叠群，我们正在对迄今为止可用的数据进行初步分析，以确定重组率是否存在相关性。在过去的一年中，已经在30个地理上不同的人群中完成了KIR和HLA分型，与佛蒙特大学的Rich Single博士合作，我们已经开始分析数据。

Sequencing of the 45kb fragment of the HLA class III region from six individuals was completed by the Sanger Center over this past fiscal year, where mini-libraries were created from each PCR fragment and multiple clones were sequenced to create contigs. Further sequencing of short stretches that were not resolved by the Sanger Center are being completed in my laboratory. Meanwhile, Beena Neelam from Dr. Bob Stephens group has begun to assemble the contigs of genomic sequences from individual clone sequences provided by the Sanger Center and we are in the process of preliminary analysis of the data available so far to see if there is a correlation with the rate of recombination.KIR and HLA typing have been completed over this past year on the 30 geographically distinct populations, and in collaboration with Dr. Rich Single at the University of Vermont, we have begun to analyze the data.