Central synaptic mechanisms regulating ovulation

促性腺激素释放激素(GnRH)神经元形成了调节生殖的最终共同途径。脉动的 下丘脑释放促性腺激素释放激素，刺激脑下垂体促性腺激素的合成和分泌。在女性身上， GnRH释放的大量激增是脑下黄体生成素的最终和先决条件信号 (Lh)高峰期，随后启动排卵。促性腺激素释放激素激增依赖于两个信号：卵巢上升 雌二醇水平和来自视交叉上核(SCN)的每日神经信号。解剖和生理数据 血管活性肠肽和加压素在昼夜节律信息传递中的支持作用 从SCN到GnRH神经元。这项研究的目标是确定潜在的神经生物学机制。 GnRH激增的产生和时间。具体地说，将使用电生理技术来探索变化 在通过测量GnRH神经元放电显示每日黄体生成素激增的动物模型中GnRH神经元的功能 GnRH神经元的突触后电流(PSCs)以及谷氨酸和γ-氨基丁酸(GABA能)。 对血管活性肠肽和加压素反应的变化将有助于阐明雌二醇反馈效应和日常信号是如何 整合、传递和接收GnRH神经元，以适当调节电涌。绝对者 促性腺激素释放激素(GnRH)激增对排卵的要求强调了阐明其确切机制的重要性 世代和时机。

Gonadotropin-releasing hormone (GnRH) neurons form the final common pathway regulating reproduction. Pulsatile GnRH release from the hypothalamus stimulates synthesis and secretion of pituitary gonadotropins. In females there is a massive surge in GnRH release that serves as the final and prerequisite signal for the pituitary luteinizinghormone (LH) surge, which subsequently initiates ovulation. The GnRH surge is dependent on two signals: rising ovarian estradiol levels and a daily neural signal from the suprachiasmatic nuclei (SCN). Anatomical and physiological data support roles for vasoactive intestinal polypeptide (VIP)and vasopressin in the transmission of circadian information from the SCN to GnRH neurons. The goal of this study is to determine the neurobiological mechanisms underlying the generation and timing of the GnRH surge. Specifically, electrophysiological techniques will be used to explore changes in GnRH neuron function in an animal model that exhibits daily LH surges through measurement of GnRH neuron firing rate and glutamatergic and gamma-aminobutyric acid (GABAergic) postsynaptic currents (PSCs) in GnRH neurons. Changes in response to VIP and vasopressin will help elucidate how estradiol feedback effects and the daily signal are integrated, transmitted to, and received by GnRH neurons for the proper regulation of the surge. The absolute requirement of the GnRH surge for ovulation underscores the importance of elucidating the exact mechanisms of its generation and timing.