CONSENT TO PARTICIPATE AS A DONOR OF PERIPHERAL BLOOD MONONUCLEAR CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 艾滋病相关：几个研究人员正在开发基于过继转移未经修饰或转基因的T细胞的新疗法，以恢复或改善宿主对病毒感染和恶性肿瘤的免疫反应。T细胞在体外生长到足以调节宿主免疫的数量，需要在培养物中添加大量的饲养细胞来提供营养和生长因子。外周血单个核细胞(PBMC)是一种良好的滋养细胞来源，是发展特异性过继免疫治疗所必需的。该方案招募正常的自愿捐献者进行白细胞分离，以获得用于开发基于免疫的疗法的PBMC。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. AIDS related: Novel therapies based on the adoptive transfer of unmodified or genetically modified T cells are being developed by several investigators to restore or improve host immune responses to viral infection and malignancy. The growth of T cells in vitro to numbers sufficient to modulate host immunity requires the addition of large numbers of feeder cells to the cultures to provide nutrients and growth factors. Peripheral blood mononuclear cells (PBMC) harvested from normal donors by leukapheresis and then subjected to gamma irradiation to prevent cell growth have been identified as an excellent source of feeder cells and are needed for the development of specific adoptive immunotherapy. This protocol enrolls normal volunteer donors for leukapheresis to obtain PBMC for use in developing immune-based therapies