PEPTIDE SYNTHESIS AND ANALYSIS EQUIPMENT: MALARIA, CANCER, DIABETES

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：申请购买自动化固相肽合成和分析设备的支持。该设备包括一个应用生物系统433A型大型多肽合成器和一个Waters模型联盟分析HPLC来评估合成肽的纯度。这些仪器在操作上是一个集成系统，是埃默里大学微化学和蛋白质组学设施生产肽所必需的。对这些仪器的要求是合理的，因为需要更换18年的大型合成器（Applied Biosystems 430A）和18年的分析HPLC (Applied Biosystems 120A)，它们不再由制造商支持。主要受益者将是隶属于13个基础和临床科学部门的29名nih支持的研究人员的主要用户组。特别强调的是1)高纯度修饰肽的合成，包括磷酸肽、脂肪酸修饰肽和具有治疗意义的肽类似物，包括抗菌肽；2)在支持的研究项目中协助多肽使用的各个方面；3)培养研究生和博士后。另外49名由美国国立卫生研究院支持的埃默里大学研究人员也将受益于该设备。除了多肽合成外，该设施还提供核酸合成、多肽和蛋白质质谱分析，以及其他生物分析和蛋白质组学服务，包括Edman测序、氨基酸分析和微分离。所要求的仪器将大大提高所描述的研究项目以及其他NIH支持的项目和埃默里研究中心的生产力。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Support is requested for the purchase of automated solid-phase peptide synthesis and analysis equipment. This equipment includes an Applied Biosystems model 433A large-scale peptide synthesizer and a Waters model Alliance analytical HPLC to evaluate the purity of the synthesized peptides. These instruments are operationally an integrated system that is essential for producing peptides at the Emory University Microchemical and Proteomics Facility. The request for these instruments is justified by the need to replace an eighteen-year-old large-scale synthesizer (Applied Biosystems 430A) and an eighteen-year-old analytical HPLC (Applied Biosystems 120A), which are no longer supported by the manufacturer. The major beneficiaries will be the Major User Group of 29 NIH-supported investigators who are affiliated with 13 basic and clinical science departments. A special emphasis is on 1) synthesis of highly purified modified peptides including phosphopeptides, fatty acid-modified peptides and peptide analogs of therapeutic interest including antibacterial peptides; 2) assistance with all aspects of peptide use in research projects supported; 3) training of graduate students and post-doctoral fellows. An additional 49 NIH-supported Emory investigators will also benefit from access to this equipment. In addition to peptide synthesis, the Facility provides nucleic acid synthesis, peptide and protein mass spectrometry, and other bioanalytical and proteomics services including Edman sequencing, amino acid analysis, and micro-separations. The requested instrumentation will significantly enhance the productivity of the research projects described as well as that of other NIH supported projects and research centers at Emory.