Amersham Biosciences Typhoon 9410

描述(由申请人提供)：本申请是购买美国生物科学公司的9410号台风。台风将作为细胞生物学、微生物学和药理学系的核心仪器，由29名研究人员组成的用户小组，几乎所有人都由NIH资助，具有广泛的研究兴趣。此外，纽约大学医学院内任何其他需要使用台风特殊能力的研究人员都将被允许加入用户组。目前在整个医学院中还没有类似的仪器。台风9410在一台机器中结合了磷成像仪、荧光图像扫描仪和发光读取器。除了amersham之外，没有一家公司销售具有同样能力的工具。此外，台风将取代目前正在使用的过时的分子动力学425磷成像仪。由于用户组中的大多数实验室已经拥有只能由amersham仪器读取的分子动力学盒式磁带，因此购买台风号是合适的，而且具有成本效益。台风还将取代老化和有限的分子动力学荧光仪575，后者目前安装在单独的建筑中。台风荧光仪比它的前身更灵敏，分辨率更高，可以检测更多种类的荧光底物。台风的检测能力不仅将提供改进的荧光测量，还将使用荧光探针标记的蛋白质和核酸杂交斑点能够定量成像，利用增强的化学发光取代用于斑点检测的X射线胶片，并能够在一维和二维聚丙烯酰胺凝胶上显示和定量用荧光染料染色的蛋白质。还要求提供资金，以获得软件许可证，以便在独立于存放仪器的设施之外的各个部门建立数据分析工作站。用户小组的一个小委员会将管理仪器及其使用的设计规则。一名训练有素的技术人员将对机器进行日常维护和监控，并对新用户进行培训。预计台风的出现将对用户群体和整个机构的研究产生重大影响。

DESCRIPTION (provided by applicant): This request is for the purchase of an Amersham Biosciences Typhoon 9410. The Typhoon will serve as a core instrument for the Departments of Cell Biology, Microbiology, and Pharmacology with a user group of 29 investigators, nearly all with NIH funding, with a wide variety of research interests. In addition, any other investigator within the NYU School of Medicine who requires the use of the special capabilities of the Typhoon will be allowed to join the user group. No similar instrument currently exists in the entire School of Medicine. The Typhoon 9410 combines a phosphorimager, a fluorescence image scanner, and a luminescence reader in one machine. No company other than Amersham markets an instrument with the same capabilities. Moreover, the Typhoon will replace an obsolete Molecular Dynamics 425 phosphorimager currently in use. Since most of the laboratories in the user group already own Molecular Dynamics cassettes, which can only be read by the Amersham instrument, the purchase of the Typhoon is appropriate and cost effective. The Typhoon will also supplant an aging and limited Molecular Dynamics Fluorimeter 575 that is currently housed in a separate building. The Typhoon fluorimeter is much more sensitive than its predecessor, offers better resolution, and can detect a wider variety of fluorescent substrates. The detection capabilities of the Typhoon will not only provide improved fluorimetric measurements but also will enable quantitative imaging of Western and nucleic acid hybridization blots labeled with fluorescent probes, replace X-ray film for detection of blots using enhanced chemiluminescence, and allow visualization and quantitation of proteins stained with fluorescent dyes on one- and two-dimensional polyacrylamide gels. Funds are also requested for a software license to set up workstations for data analysis in the individual departments separate from the facility housing the instrument. A small committee of the user group will administer the instrument and design rules for its use. A trained technician will maintain and monitor the machine on a daily basis and educate new users. It is anticipated that the availability of the Typhoon will have great impact on the research of the user group and the institution as a whole.