A HIGH-SPEED CELL SORTER FOR A MULTI-USER CORE FACILITY: HEMATOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：宾夕法尼亚州立大学定量细胞分析中心寻求资金购买高速细胞分选机。该中心是哈克生命科学研究所的共享技术设施之一，为整个大学公园校园提供服务。九个主要的NIH支持的研究人员寻求一种仪器，将排序至少比设施的现有分拣机快五倍，由于分拣的速度，提高了可行性水平。这种多参数仪器还将通过允许多个标记物的更多同时分析来提高分析能力，并且由于改进的光学配置而提高灵敏度。它还将有能力执行CFP/YFP FRET应用。目前，我们只能在我们的成像系统上做到这一点。三位分子毒理学家将对肝细胞进行分类，以进行后续分子分析和信号特征分析。用户组中的五名免疫学家将对罕见事件群体（<1%）进行分类，这对于当前的仪器来说是困难的（没有足够的回收细胞用于所需的实验）。应用包括涉及哮喘的免疫亚群的分选、红系祖细胞的分选、HIV克隆的分选和涉及肠易激性疾病的T细胞亚群的分选。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Center for Quantitative Cell Analysis of the Pennsylvania State University seeks funds to purchase a high-speed cell sorter. This center is one of the Shared Technology Facilities of the Huck Institute of the Life Sciences, providing services for the entire University Park campus. Nine major NIH supported researchers seek an instrument that will sort at least five times faster than the facility's existing sorter with an increased level of viability due to the speed of the sorting. This multiparameter instrument will also increase analytical proficiency by allowing more simultaneous analysis of multiple markers and increased sensitivity because of improved optical configuration. It will also have the capacity to perform CFP/YFP FRET applications. Presently we can only do this on our imaging systems. Three molecular toxicologists will sort hepatocytes for subsequent molecular analysis and signaling characteristics. Five immunologists in the user group will sort rare event populations (<1%), which is difficult with the current instrument (not enough recovered cells for the needed experiments). Applications include sorting of immune subsets involved in asthma, sorting of erythroid progenitors, sorting HIV clones and sorting T cell subsets involved in Irritable Bowel Disease.