A HIGH-SPEED CELL SORTER FOR A MULTI-USER CORE FACILITY: ASTHMA

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：宾夕法尼亚州立大学定量细胞分析中心寻求资金购买高速细胞分选机。该中心是哈克生命科学研究所的共享技术设施之一，为整个大学园区提供服务。美国国立卫生研究院支持的九个主要研究人员正在寻找一种仪器，这种仪器的分拣速度至少是该设施现有分拣机的五倍，由于分拣的速度，其可行性水平也有所提高。这种多参数仪器还将通过允许对多个标记进行更多的同时分析并由于改进的光学配置而提高灵敏度，从而提高分析熟练程度。它还将具有执行CFP/YFP FRET应用的能力。目前，我们只能在我们的成像系统上做到这一点。三位分子毒物学家将对肝细胞进行分类，以进行后续的分子分析和信号特征。用户组中的五位免疫学家将对罕见事件人群(1%)进行分类，这在目前的仪器中是困难的(没有足够的回收细胞来进行所需的实验)。应用包括对与哮喘有关的免疫亚群的分类、对红系祖细胞的分类、对HIV克隆的分类以及对与肠易激疾病有关的T细胞亚群的分类。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Center for Quantitative Cell Analysis of the Pennsylvania State University seeks funds to purchase a high-speed cell sorter. This center is one of the Shared Technology Facilities of the Huck Institute of the Life Sciences, providing services for the entire University Park campus. Nine major NIH supported researchers seek an instrument that will sort at least five times faster than the facility's existing sorter with an increased level of viability due to the speed of the sorting. This multiparameter instrument will also increase analytical proficiency by allowing more simultaneous analysis of multiple markers and increased sensitivity because of improved optical configuration. It will also have the capacity to perform CFP/YFP FRET applications. Presently we can only do this on our imaging systems. Three molecular toxicologists will sort hepatocytes for subsequent molecular analysis and signaling characteristics. Five immunologists in the user group will sort rare event populations (<1%), which is difficult with the current instrument (not enough recovered cells for the needed experiments). Applications include sorting of immune subsets involved in asthma, sorting of erythroid progenitors, sorting HIV clones and sorting T cell subsets involved in Irritable Bowel Disease.