DNA Vaccines

我们优化了艾滋病的DNA疫苗程序，并最终优化了其他应用程序。我们通过对抗原形式、表达和递送的优化来达到最优的结果。优化的DNA载体在预防和治疗性疫苗接种感染SIV的恒河猴中显示出对高病毒血症的保护作用。SIV是最忠实的艾滋病灵长类动物模型。我们在合作研究中使用了分子佐剂来提高DNA疫苗的免疫应答。我们已经证明，IL-12提高了猕猴对SIV DNA载体的体液免疫和细胞免疫应答。我们还表明，优化后的DNA在DNA初始病毒载体Boost中具有很好的免疫启动作用。HIV优化的载体正在进行临床试验。我们实验室的几种载体组合的开发为艾滋病和癌症的更多临床应用提供了机会。

We optimize DNA vaccine procedures for AIDS and eventually other applications. We used optimization of antigen form, expression and delivery to achieve optimal results. The optimized DNA vectors were shown to provide protection from high viremia in both prophylactic and therapeutic vaccination of rhesus macaques infected by SIV, the most faithful primate model for AIDS. We have used molecular adjuvants to increase immune response to DNA vaccination in collaborative studies. We have shown that IL-12 increased both humoral and cellular immune response to SIV DNA vectors in macaques. We have also shown that the optimized DNA is excellent in priming immune response in DNA prime-virus vector boost. The HIV optimized vectors are in clinical trials. The development of several vector combinations from our lab provides the opportunity for additional clinical applications in AIDS and cancer.