ARRAY

Array Core提供阵列和相关服务，用于分析DMA拷贝数变化（阵列 CGH）以及自定义阵列打印。 阵列CGH：用于CGH分析的阵列由分布在人和小鼠中的BAG克隆组成。 基因组这些阵列允许可靠地检测来自二倍体的单拷贝变化以及高水平的 扩增。BAG阵列从福尔马林固定石蜡包埋的样品中产生良好的数据， 允许目前无法使用其他来源的阵列进行的研究。核心将继续提供 用于人和鼠分析的分辨率为约1 - 1.5 Mb的BAG阵列，以及32，000 BAG的全人类 基因组平铺阵列。后者允许分辨率为BAG长度的一小部分，或远低于100 kb。在 除了这些标准数组，Core还将为各个项目打印自定义数组， 关于BAG阵列的使用（DMA分离、标记、杂交、成像和分析），并咨询 对数据的解释。它还将继续提供完整的阵列处理服务，从 调查人员和返回数据。 仪器：核心操作三个阵列成像系统，一个Axon 4000 B激光扫描仪，一个定制的 内置100万像素CCD摄像系统和定制的400万像素CCD成像系统。核还 操作一台定制的高速阵列打印机其他设备包括一个液体处理机器人和一个完全 用于阵列处理和制备打印溶液的储备实验室。

The Array Core provides arrays and associated services for analysis of DMA copy number variation (array CGH) as well as custom array printing. Array CGH: The arrays for CGH analysis consist of BAG clones distributed over the human^and mouse genomes. These arrays allow reliable detection of single copy changes from diploid as well as high-level amplifications. The BAG arrays produce good data from formalin fixed-paraffin embedded samples and thus allow studies that are not currently possible with arrays from other sources. The core will continue to offer BAG arrays with -1-1.5 Mb resolution for human and murine analysis, and a 32,000 BAG whole human genome tiling array. The latter allows resolution to a fraction of the length of a BAG, or well below 100kb. In addition to these standard arrays, the Core will print custom arrays for individual projects, provide instruction on use of the BAG arrays it offers (DMA isolation, labeling, hybridization, imaging and analysis), and consult on interpretation of data. It will also continue to offer a full array processing service, receiving DNA from investigators and returning data. Instrumentation: The Core operates three array imaging systems, an Axon 4000B laser scanner, a custom built 1M pixel CCD camera system, and a custom built 4M pixel CCD imaging system. The Core also operates a custom, high-speed array printer. Other equipment includes a liquid handling robot and a fully stocked laboratory for array processing and preparation of printing solutions.