PROTEIN MICROARRAY CORE

拟议的蛋白质微阵列核心(PMC)实验室的目标是在Keck DNA微阵列资源的现有专业知识的基础上，通过使用蛋白质微阵列将高通量并行分析的能力带入耶鲁大学和其他地方的神经蛋白质组研究领域。能够在相对较低的浓度下量化数百种预先选择的蛋白质的相对表达水平，将为蛋白质和脂肪分离和图谱核心所提供的技术提供强大而独特的补充。微阵列资源是一个功能齐全的DNA微阵列制造实验室，拥有训练有素的工作人员和高通量的机器人分配器、阵列、扫描仪和软件。所有这些现有的基础设施和经验将被改编和/或复制用于蛋白质阵列应用，这是一项目前不受凯克实验室或耶鲁大学任何其他核心实验室支持的技术。为了满足这一需求，蛋白质微阵列核心将开发和实施一个强大的、经过验证的、具有成本效益的平台，用于制造蛋白质微阵列，其蛋白质靶标集中在神经科学研究上。

The goal of the proposed Protein Microarray Core (PMC) laboratory is to build on the current expertise within the Keck DNA Microarray Resource to bring the power of high throughput parallel assays into the realm of neuroproteomics research at Yale and beyond via the use of protein microarrays. The ability to quantify the relative level of expression of hundreds of pre-selected proteins at comparatively low concentrations will provide a powerful and unique complement to the technologies that will be made available by the Protein and Lipid Separation and Profiling Core. The Microarray Resource is a fully functional DNA microarray fabrication laboratory with trained staff and high throughput robotic dispensers, arrayers, scanners and software in place. All of this existing infrastructure and experience will be adapted and/or duplicated for use in protein array applications, which is a technology that is currently not supported by the Keck Laboratory nor any other core laboratory at Yale. To meet this need, the Protein Mieroarray Core will develop and implement a robust, validated, cost effective, platform for fabrication of protein microarrays with protein targets focused on neuroscience research.