TESTING GHRELIN AND PYY VACCINES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Ghrelin是一种修饰的肽，似乎在动物中引起饥饿反应。我们已经合成了两种基于ghrelin的疫苗，它们在小鼠中引起了特异性抗体反应，而没有任何有害影响。我们还从这些动物中产生了抗胃饥饿素的单克隆抗体。针对生长激素释放肽的疫苗具有控制食欲的潜力，因此可以为人类肥胖提供有益的治疗。因此，重要的是要发现这些疫苗是否会在非人灵长类动物中引发免疫应答。 PYY 3 -36是一种抑制动物饥饿反应的肽。我们将合成一种基于PYY的疫苗，并对其进行测试，看看它是否会在小鼠中引发特异性抗体反应，而不会产生任何有害影响。我们还将从这些动物中生产抗PYY单克隆抗体。针对PYY的疫苗具有控制食欲的潜力，因此可以为人类厌食症或食欲不振提供有益的治疗。因此，重要的是要发现这种疫苗是否会在非人灵长类动物中引发免疫应答。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ghrelin is a modified peptide that appears to elicit a hunger response in animals. We have synthesized two ghrelin-based vaccines that have elicited specific antibody responses in mice without any harmful effects. We have also produced anti-ghrelin monoclonal antibodies from these animals. A vaccine directed against ghrelin has potential for controlling appetite and therefore could provide a beneficial treatment for obesity in humans. Therefore it is important to discover if these vaccines will elicit an immune response in non-human primates. PYY3-36 is a peptide that suppresses the hunger response in animals. We will synthesize a PYY-based vaccine and test it to see if it will elicit a specific antibody responses in mice without any harmful effects. We will also produce anti-PYY monoclonal antibodies from these animals. A vaccine directed against PYY has potential for controlling appetite and therefore could provide a beneficial treatment for anorexia or poor appetite in humans. Therefore it is important to discover if this vaccine will elicit an immune response in non-human primates.