ANIMAL MODEL CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目的：本项目对HIV潜伏机制和病毒库进行全面分析。组织培养系统的体外研究（项目1、2和3）将与该核心的体内研究（“非人类灵长类动物的HIV/SIV储存库和潜伏期”）密切协调。因此，该核心将为本项目的动物模型研究提供恒河猴和适当的基础设施支持。目标1：确定SIV/猕猴和RT-SHIV/猕猴系统的条件，以分析在HIV感染者中观察到的病毒潜伏期/库；目的2：分析接受抗逆转录病毒治疗的受感染猕猴体内的病毒定位；目的3：确定病毒和宿主细胞因子在病毒潜伏期、持久性和再激活调控中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: This Program Project conducts a comprehensive analysis of the mechanism(s) of HIV latency and viral reservoirs. In vitro investigations in tissue culture systems (Projects 1, 2, and 3) will be closely coordinated with in vivo studies in this core ("Non-human Primates for HIV/SIV Reservoirs and Latency"). Accordingly, this core will provide rhesus macaques and appropriate infrastructure support required for the animal model studies in this Program Project. Three Specific Aims address this hypothesis: Aim 1: to define conditions in the SIV/macaque and RT-SHIV/macaque systems for analysis of viral latency/reservoirs as observed in humans infected with HIV; Aim 2: to analyze virus localization in infected macaques undergoing antiretroviral therapy; Aim 3: to determine the role of viral and host-cell factors in the regulation of viral latency, persistence, and reactivation.