MRI ASSESSMENT OF LUNG PERFUSION AFTER RADIATION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。本研究的目的是检测电离辐射后肺灌注和肺中可能的质子密度的变化。我们将使用尾静脉输注magnavist比较对照和照射大鼠肺（全肺，单剂量照射）中的血液灌注和血管内皮渗漏。将通过右肺和左肺之间的信号差异确定辐照肺和对照肺之间的灌注/血容量比较。将使用确定时间段内的图像序列分析造影剂泄漏和积聚。还可以计算辐照的和未辐照的肺之间的质子密度差。最终将在照射后的10个不同时间点对动物进行成像，每个时间点对3至4只动物进行成像。研究的第一步将是在照射后挑选三到四个时间点，以确定成像方案的有效性，并根据需要进行调整。将进一步讨论适当的成像技术和序列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A goal of this study is to detect changes in lung perfusion and possibly proton density in lungs after ionizing radiation. We will compare blood perfusion and leakage of vascular endothelium in control and irradiated rat lungs (whole lung, single dose irradiation) using tail vein infusion of magnavist. A comparison of perfusion/blood volume between irradiated and control lungs will be determined by signal difference between right and left lungs. A sequence of images over a determined time period will be used to analyze leakage and build up of contrast agent. Proton density difference between irradiated and un-irradiated lungs may also be calculated. Animals will eventually be imaged at 10 different timepoints after irradiation, with three to four animals imaged at each timepoint. The first step in the study will be to pick out three to four timepoints after irradiation to determine effectiveness of the imaging protocol and make adjustments as needed. Appropriate imaging techniques and sequences to be used will be discussed further.