FLASH/REASH LABELING OF TETRACYSTEINE-TAGGED PROTEINS IN ZEBRAFISH

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该项目的目标是熟悉标记斑马鱼胚胎的技术方面，斑马鱼胚胎表达基因改变后含有四胱氨酸标签的蛋白质。该标签可被市售荧光团FlAsH-EDT2和ReAsH-EDT2识别。任何一个荧光团与四胱氨酸标签结合都会产生荧光信号，该荧光信号可以将二氨基联苯胺光转化为亲锇沉淀物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to become familiar with the technical aspects of labeling zebrafish embryos that express proteins genetically altered to contain a tetracysteine tag. This tag is recognized by the commercially available fluorophores FlAsH-EDT2 and ReAsH-EDT2. Binding of either fluorophore to the tetracysteine tag results in a fluorescent signal that can photoconvert diaminobenzidine into an osmiophilic precipitate.