PROTEIN CONFORMATIONAL CHANGE TRIGGERED BY NI-BCHL-A EXCITATION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们计划研究光激发后Ni-BCHL-a在从头模型中的结构变化和动力学机制。因此，模板是具有蛋白质灵活性的纯合成多肽结构，在分子水平上量身定做，以完成天然蛋白质所展示的各种化学、机械和电子功能，但具有生物体所不具备的简单性和健壮性。从前人对Ni-BCHL-a络合物的光谱研究可知，在光激发后，轴向配体发生排斥。我们想用这个光触发器来启动从头蛋白质中的蛋白质运动，以研究蛋白质构象动力学。超快瞬时吸收光谱将被用来进一步研究光激发后发生的结构变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We plan to study structural changes and kinetic mechanism after photoexcitation of a Ni-BChl-a incorporated into a de-novo maquette. A maquette is thereby a purely synthetic peptide structures with protein flexibility that is tailored at the molecular level to accomplish as wide a variety of chemical, mechanical and electrical functions as displayed by natural proteins, but with a simplicity and robustness uncharacteristic of living matter. From previous studies on the optical spectroscopy of Ni-BChl-a complexes, it is known that a repulsion of the axial ligands occurs after photoexcitation. We would like to use this phototrigger to initiate protein motion in a de-novo protein to study protein conformational dynamics. Ultrafast transient absorption spectroscopy is to be used to further investigate into structural changes occurring after photoexcitation.