K+ SYMPORTER

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们带来了一种共同运输K+和Na+的膜蛋白的晶体。目前还没有已发表的Na/K转运体的结构，这一结构将有助于揭示离子共转运的机制。最近我们在国际象棋上测试晶体，我们发现了一些晶体衍射到~4A的条件。我们一直在优化条件，需要访问以检查新晶体并收集完整的数据集。由于衍射图的薄弱，国内来源的数据采集已被证明是不可能的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are bringing crystals of a membrane protein that co-transports K+ and Na+. There are no published structures of Na/K-symporters and this structure would shed light into the mechanism of ion co-transport. Recently we were at CHess testing crystals and we found some conditions with crystals that diffract to ~4A. We have been optimizing conditions and need access to check the new crystals and collect full data sets. Data collection at home source has proved to be impossible due to do the weakness of the diffraction pattern.