MYOCELLULAR ENERGY METABOLISM: STUDIES USING SELF-REFERENCING MICROELECTRODES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。哈珀博士的研究重点是肌肉葡萄糖转运蛋白的分子生理学，因为它与II型糖尿病有关。这种组织内的葡萄糖隔离不足被认为是疾病进展的一个明显的并发症。在此之前，测量葡萄糖转运既复杂又缓慢。然而，由BioCurrents研究中心开发的自参照葡萄糖微型传感器的出现，极大地提高了我们在单细胞水平上检查运输动力学的能力--这是哈珀博士实验室研究的主题。为了促进这项研究，在渥太华的哈珀博士的实验室里安装了一个完整的安培系统。该团队现在完全能够独立操作该系统，并能够构建自己的感应器。今年，她的系统进行了全面检修，包括安装了更新的软件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr Harper's studies focus on the molecular physiology of the muscle glucose transporter as it relates to Type II diabetes. Insufficient glucose sequestration within this tissues is accepted as one clear complication of the disease progression. Measuring glucose transport has previously been complicated and slow. However, the advent of the self-referencing glucose microsensor, developed by the BioCurrents Research Center, has greatly improved our ability to examine the dynamics of transport at the single cell level - the subject of the studies in Dr Harper's laboratory. To facilitate this research a full amperometric system was installed in Dr Harper's laboratory in Ottawa. The group is now fully capapble of operating the system independently and able to build their own sensrors. This year saw a complete overhaul of her system including the installation of updated software.