COPPER AND IRON HOMEOSTASIS IN YEAST

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。铜和铁是所有需氧生物在许多生物过程中所必需的两种必需营养素，包括能量产生、防止氧化应激和氧气运输等。从酵母菌到哺乳动物，铜和铁的稳态机制都是高度保守的，我们对这些营养物质在后生动物中的吸收和分布机制的了解大多来自于对酵母菌的研究。我们实验室的一个研究领域是旨在了解酵母细胞如何通过这种金属从细胞外环境跨质膜的协调运输获得铜，以及铜跨细胞膜的动员。我们和其他实验室的证据表明，酵母中需要特定的金属还原酶来高亲和力地摄取铜和铁，但这些金属还原酶与运输蛋白的功能和潜在物理相互作用的细节目前尚不清楚。本研究拟利用FRET研究酵母质膜金属还原酶Fre1与铜转运体Ctr1之间潜在的物理相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Copper and iron are two essential nutrients required by all aerobic organisms for numerous biological processes including energy generation, protection from oxidative stress, and oxygen transport, among others. The mechanisms of copper and iron homeostasis are highly conserved from yeast to mammals, and much of what is known about the mechanisms of uptake and distribution of these nutrients in metazoans has come from studies in yeast. One area of investigation in our lab is aimed at understanding how yeast cells acquire copper through the coordinated transport of this metal from the extracellular environment across the plasma membrane, as well as the mobilization of copper across intracellular membranes. Evidence from our lab and others suggests that specific metalloreductases are required for high-affinity copper and iron uptake in yeast, but the details of the functional and potential physical interaction of these metalloreductases with the transport proteins are currently not understood. Here we propose to investigate the potential physical interaction between the plasma membrane metalloreductase Fre1 and the copper transporter Ctr1 in yeast using FRET.