POLARIZED MORPHOGENESIS: ROLE OF ZDS PROTEINS IN S CEREVISIAE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在任何真核生物中，对细胞如何以不对称或极化的方式生长的充分理解仍然是未知的，这是生命和/或繁殖物种的能力所必需的过程。迄今为止，大多数研究都集中在了解启动和维持极化细胞生长的细胞内信号事件。本文提出的研究考察了这一细胞过程的反面。酿酒酵母（贝克酵母）是一种低细胞复杂性的真核生物，它的芽形成被用来识别负调控极化细胞生长的细胞内信号事件。特别注意的是，在酿酒酵母中，由蛋白质与Zds蛋白相互作用引起的信号事件，这是负调节极化细胞生长所必需的。与Zds2p相互作用调节极化细胞生长的蛋白通过基于候选的方法进行鉴定，该方法利用差分双杂交分析。该分析结果是与酵母资源中心合作进行的，将用于指导后续的生化和细胞学分析，旨在破译真核生物极化细胞生长的机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Still unknown in any eukaryote is a full understanding of how cells grow in an asymmetric or polarized manner, a process essential for life and/or the ability to propagate species. To date, most research has focused on understanding the intracellular signaling events that initiate and maintain polarized cell growth. The research proposed herein examines the opposite side of this cellular process. Bud formation in S. cerevisiae (Baker¿¿s yeast), a eukaryote of low cellular complexity, is used to identify intracellular signaling events that negatively regulate polarized cell growth. Particular attention is given to signaling events that result from protein interactions with Zds proteins, which are essential for negatively regulating polarized cell growth, in S. cerevisiae. Proteins that interact with Zds2p to regulate polarized cell growth are identified with a candidate-based approach that utilizes differential two-hybrid analysis. The results from this analysis, performed in collaboration with the Yeast Resource Center, will be used to direct subsequent biochemical and cytological analyses aimed at deciphering the mechanisms of polarized cell growth in eukaryotes.