TWO-HYBRID SCREENING WITH POTENTIAL CHROMOSOME SEGREGATION PROTEINS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的实验室研究了芽殖酵母中的染色体分离，重点是动粒，这是一种介导DNA和纺锤体之间相互作用的大蛋白质复合物。我以前曾与酵母资源中心合作，用动粒蛋白进行双杂交筛选，并发表了一篇论文。我在自己的实验室继续研究动粒，重点是Ndc 80复合物，这是一种高度保守的复合物，是纺锤体检查点功能所必需的。我的实验室已经采取了生物化学和遗传学的方法来识别与Ndc 80复合物相互作用的基因和途径。我们已经确定了两个基因，可能有作用的动粒功能，并希望确定相互作用的蛋白质，使用全基因组双杂交筛选。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab studies chromosome segregation in budding yeast with a focus on the kinetochore which is a large protein complex that mediates interaction between DNA and the spindle. I have previously collaborated with the Yeast Resource Center to perform two-hybrid screens with kinetochore proteins, which resulted in a publication. I have continued my studies of the kinetochore in my own lab, with a focus on the Ndc80 complex, which is a highly conserved complex that is required for spindle checkpoint function. My lab has taken a biochemical and genetic approach to identify genes and pathways that interact with the Ndc80 complex. We have identified two genes that may have a role in kinetochore function and would like to identify interacting proteins using genome-wide two-hybrid screening.