THE STRUCTURE OF RNA POLYMERASE II AND ASSOCIATED FACTORS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。RNA聚合酶II(PolII)是基因表达的中心酶，在真核生物中合成所有的mRNA。PolII转录的复杂调控是细胞生长和分化的基础。POL II既是一台能够解开DNA、RNA聚合和校对的机器，也是一个组装更大的起始和延伸复合体的平台。最近对酿酒酵母(Pol II)10个亚基0.5MDAL的结构测定(分辨率为2.8？)和转录的PolII复合体(分辨率为3.3？)，为许多方面的研究开辟了道路。可以解决PolII与相互作用分子共晶体的结构，可以充分利用定点突变的力量来理解转录机制，等等。PolII转录机制的结构确定对于理解转录的机制和调控是必不可少的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA polymerase II (pol II), the central enzyme of gene expression, synthesizes all mRNA in eukaryotes. The intricate regulation of pol II transcription underlies cell growth and differentiation. Pol II is both a machine, capable of DNA unwinding, RNA polymerization, and proofreading, and also a platform for the assembly of even larger initiation and elongation complexes. The recent structure determination of ten-subunit, 0.5 mDal, Saccharomyces cerevisiae (pol II) at 2.8 ¿¿resolution, and of a transcribing pol II complex at 3.3 ¿¿resolution, opens the way to many lines of investigation. Structures of cocrystals of pol II with interacting molecules can be solved, the full power of site-directed mutagenesis can be brought to bear on understanding the transcription mechanism, and so forth. Structure determination of the pol II transcription machinery is essential for understanding the mechanism and regulation of transcription.