CRYSTAL STRUCTURE OF COLB

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。所有的大肠杆菌素都可以排列成三个功能结构域。氨基末端区域是跨外膜转运所必需的。中心结构域是识别外膜受体所必需的，羧基末端结构域含有大肠杆菌素的杀伤活性。这些杀伤功能的范围从核酸内切酶到抑制肽聚糖合成，再到通过通道形成使细胞质膜失能。大肠杆菌素B通过FepA进入细胞，通过在细胞质膜中形成通道杀死细胞。我的实验室对形成通道的大肠杆菌素的杀伤作用感兴趣。已经确定了许多关于各种大肠杆菌素的通道形成结构域在脂质双层中的体外行为的方法。对于B族大肠杆菌素，在体内进行的工作很少，并且没有任何晶体结构的益处来指导理解和诱变。因此，大肠杆菌素B是结晶和我们进一步研究的极好候选物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. All colicins can be arranged into three functional domains. The amino terminal region is required for translocation across the outer membrane. The central domain is required for recognition of the outer membrane receptors and the carboxy terminal domain contains the killing activity of the colicin. These killing functions range from endonucleases to inhibition of peptidoglycan synthesis, to deenergizing cytoplasmic membranes by channel formation. Colicin B enters the cell through FepA, kills cells by forming channels in the cytoplasmic membrane. My lab is interested in the killing action of the channel-forming colicins. Much has been determined about the means by which the channel-forming domains of various colicins behave in vitro in lipid bilayers. With B-group colicins, very little work has been done in vivo, and that without benefit of any crystal structures to guide understanding and mutagenesis. Colicin B is thus an excellent candidate for crystallization and our further studies.