THE STRUCTURE OF SOXAX: A CYTOCHROME WITH A ROLE IN THIOSULFATE OXIDATION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。还原性硫化合物可被多种微生物用作电子供体，支持自养生长。硫代硫酸盐是自然界中还原硫最丰富的形式之一，许多微生物都有氧化这种化合物的能力。研究得最好的硫代硫酸盐氧化途径是硫代硫酸盐-氧化多酶系统（TOMES），它可以以细胞色素c作为电子受体将硫代硫酸盐、亚硫酸盐或硫化物氧化成硫酸盐。该途径由硫氧化（sox）基因簇编码，包含血红素蛋白（SoxB, SoxC）以及许多细胞色素（SoxA, SoxX, SoxD, SoxE）。SoxAX是一种异二聚体蛋白，具有两个分别约为30和15 kDa的含血红素c亚基。这里要研究的SoxAX是来自斯塔基亚的小说

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reduced sulfur compounds can be used a electron donors by a variety of microorganisms, supporting autotrophic growth. Thiosulfate is one of the most abundant forms of reduced sulfur in Nature, and the ability to oxidise this compound is found in many microorganisms. The best studied pathway of thiosulfate oxidation involves the thiosulfate-oxidising multi-enzyme system (TOMES) which can oxidise thiosulfate, sulfite or sulfide to sulfate with cytochrome c as the electron acceptor. This pathway is encoded by sulfur oxidation (sox) gene clusters and contains heme proteins (SoxB, SoxC) as well as a number of cytochromes (SoxA, SoxX, SoxD, SoxE). SoxAX is heterodimeric protein with two heme c-bearing subunits of approximately 30 and 15 kDa, respectively. The SoxAX to be studied here is that from Starkeya novell