IN VITRO APPROACH TO PROBLEMS OF CLINICAL ALLERGY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本研究的目的是进一步确定具有阳性病史和皮肤试验的个体对挑战性刺痛有反应的百分比，以及单个刺痛是否足够的问题。该研究的另一个方面涉及尝试确定预测反应的参数，因此不再需要挑战刺穿。其中大多数将是实验室研究，只需要抽血。总之，本研究的好处是在临床控制环境下监测这些反应，开发更准确的昆虫叮咬过敏标志物，以检测易发生严重反应的个体，并避免许多患者不必要的治疗。在2005年，我们继续对毒液皮肤测试呈阳性和/或RAST血液测试呈阳性的昆虫过敏患者进行刺痛挑战。有4个故意刺痛挑战，分为以下组：2个为常规刺痛挑战，2个为皮肤试验/RAST阴性刺痛挑战。在2005年的刺痛挑战中，主要包括4名新患者。2例皮肤试验/病史阳性患者中，1例无全身性反应，1例有中度严重反应，不危及生命，用药后容易逆转。该患者需要肾上腺素治疗，效果良好。皮肤试验/RAST阴性：2002年，有2例患者出现刺痛挑战，1例无反应，1例可疑反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to further define what percentage of individuals with a positive history and skin test will react to a challenge sting, along with the question of whether a single sting is adequate. Another aspect of this study involves an attempt to determine parameters predictive of a reaction, so that challenge stings are no longer necessary. Most of these will be laboratory studies, which require only the drawing of blood. In conclusion, the benefits of this study are to monitor these reactions in a controlled clinical setting, to develop more accurate markers for insect sting allergy to detect the individuals who are susceptible to severe reactions, and to avoid unnecessary therapy in many patients. During the year 2005, we continued sting challenging insect allergic patients with positive venom skin tests and/or positive RAST blood testing. There were 4 deliberate sting challenges with the following groups: 2 were regular sting challenges, and 2 were skin test/RAST negative sting challenges. The sting challenge in the year 2005, consisted of 4 new patients in the primary. Of the 2 stings of the patients with positive skin test/histories, there was 1 with no systemic reactions, and 1 patient with a moderately severe reaction which were not life-threatening and was easily reversed with medications. This patient required Epinephrine treatment, haing a good response. Skin Test/RAST negative: During the year 2002, there were 2 patients who had sting challenges with 1 having no reaction and 1 a questionable reaction.