Macromolecular metabolosome formation associated with 12-propanediol utilisation

丙二醇是几种植物糖发酵的结果，在肠道中浓度相当高。许多肠道细菌，如肠沙门氏菌，有能力利用丙二醇作为碳源。然而，这种代谢的非凡之处在于，这些细菌实际上制造了自己的蛋白质细胞器，或微室，来容纳这种代谢活动。蛋白质细胞器被称为代谢体，它的形成与大约20种蛋白质有关，其中一些形成衣壳的外壳，一些与代谢酶有关，一些与运输，支架和调节有关。丙二醇利用基因与钴胺素生物合成基因是连续的，并相互调节，这表明丙二醇分解代谢是许多细菌从头合成B12的主要原因。在这个应用中，我们希望通过在分子水平上对细胞器的研究来研究丙二醇代谢体的形式和功能。本应用程序的目的是研究丙二醇代谢体。我们计划研究这20种左右的蛋白质在代谢过程中所起的作用，包括它们的功能和定位。此外，我们将研究代谢体相对于单个酶的生产的代谢优势。最后，我们将探讨代谢体的结构方面，包括运输元素进出大分子复合物的能力

Propanediol is produced as a result of the fermentation of several plant sugars and it is found in quite high concentrations in the intestine. A number of enteric bacteria, such as Salmonella enterica, have the ability to utlise propanediol as a carbon source. However, what is remarkable about this metabolism is that these bacteria actually make their own proteinaceous organelle, or microcompartment, to house this metabolic activity. The proteinaceous organelle is called a metabolsome and its formation is associated with about twenty proteins, some of which form the shell of the capsid, some of which are associated with the metabolic enzymes and some of which are associated with transport, scaffolding and regulation. The genes for propanediol utilisation are contiguous and coregulated with the cobalamin biosynthetic genes, indicating that propanediol catabolism is the primary reason for de novo B12 synthesis in many bacteria. In this application we wish to study the form and function of the propanediol metabolosome by initiating research into the organelle at the molecular level. The aim of this application is to investigate the propanediol metabolosome. We plan to study the role played by the twenty or so proteins in the metabolic process, including their function and localisation. Moreover, we will investigate the metabolic advantage of the metabolosome over the production of individual enzymes. Finally, we will explore structural aspects of the metabolosome including the ability to transport elements into and out of the macromolecular complex