Proteomic-based Approach to Ventilator Associated Pneumonia

描述（由申请人提供）：这项拟议的职业中期研究者奖的目标是通过加强指导和扩大以患者为导向的研究计划，在肺和危重病科扩大以患者为导向的转化研究培训。Schnapp继续建立她以病人为导向的研究计划（检查肺损伤和感染的界面），并确保有足够的保护时间，通过将他们融入这个计划，为指导初级教师和研究员提供更多的机会。此外，它将为Schnapp博士参与额外的课程工作提供必要的保护时间，以进一步加强她作为以患者为导向的研究人员的自身发展。该研究计划的目的是分析呼吸机相关性肺炎（VAP）时的空气环境，以期深入了解VAP的致病机制，了解ARDS患者对感染的反应，提高高危患者VAP的诊断水平。这些信息可能会建议新的治疗方式的基础上确定的途径，或建议额外的验证性诊断标准的VAP。该项目建立在目前资助的项目基础上，包括NIH SCCOR急性肺损伤项目和新资助的RO1，以开发蛋白质组学方法来分析肺损伤期间的支气管肺泡灌洗液。指导计划扩大了Schnapp博士在指导年轻医生进行以患者为导向的研究方面的积极作用，以及她在为学员开发转化研究课程方面的领导作用。研究和指导计划将利用华盛顿大学的优秀资源和基础设施，包括肺科与公共卫生学院的长期互动，现有的K30和K12计划以及最先进的蛋白质组学设施。华盛顿大学为肺部医学的合作培训和以患者为导向的研究提供了出色的环境。

DESCRIPTION (provided by applicant): The goal of this proposed midcareer investigator award is to expand training in patient-oriented translational research within the Pulmonary and Critical Division through enhanced mentoring and expansion of patient-oriented research program. This proposal will provide sufficient resources for Dr. Schnapp to continue to build her patient-oriented research program (examining the interface of lung injury and infection) and to insure sufficient protected time to provide more opportunities for mentoring junior faculty and fellows by integrating them into this program. It addition, it will provide the necessary protected time for Dr Schnapp's participation in additional coursework to further enhance her own development as a patient-oriented researcher. The goal of the research plan is to analyze the airspace environment during ventilator associated pneumonia (VAP) in hopes of obtaining insight into the pathogenic mechanisms of VAP, provide insight into the host response to infection in the setting of ARDS and improve the diagnosis of VAP in high-risk patients. This information may suggest new therapeutic modalities based on identified pathways, or suggest additional confirmatory diagnostic criteria for VAP. This program builds on currently funded programs, including NIH SCCOR program in Acute Lung injury, and newly funded RO1 to develop proteomic methodologies to analyze the bronchoalveolar lavage fluid during lung injury. The mentoring plan expands Dr. Schnapp's very active role in mentoring of young physicians in patient-oriented research and her leadership role in developing a curriculum in translational research for trainees. The research and mentoring program will take advantage of outstanding resources and infrastructure at the University of Washington, including long standing interactions of the Pulmonary Division with the School of Public Health, existing K30 and K12 programs, and state-of- the-art proteomic facilities. The University of Washington provides an outstanding setting for collaborative training and patient-oriented research in Pulmonary Medicine.